The Vicia faba leghemoglobin gene VfLb29 is induced in root nodules and in roots colonized by the arbuscular mycorrhizal fungus Glomus fasciculatum

• Published in: Molecular plant-microbe interactions Vol. 10; no. 1; pp. 124 - 131
• Main Authors: Fruhling, M. (Universitat Bielefeld, Bielefeld, Germany.), Roussel, H, Gianinazzi-Pearson, V, Puhler, A, Perlick, A.M
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.01.1997; The American Phytopathological Society
• Subjects: ADN; Agronomy. Soil science and plant productions; Amino Acid Sequence; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Biological and medical sciences; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Economic plant physiology; EXPRESION GENICA; EXPRESSION DES GENES; Fabaceae - genetics; Fabaceae - metabolism; Fabaceae - microbiology; Fundamental and applied biological sciences. Psychology; GENE; Gene Expression Regulation, Plant; Gene Library; GENES; GLOMUS FASCICULATUM; LEGAHEMOGLOBINA; Leghemoglobin - biosynthesis; Leghemoglobin - genetics; LEGHEMOGLOBINE; MICORRIZAS ARBUSCULARES VESICULARES; Molecular Sequence Data; Mucorales - physiology; MYCORHIZE A VESICULE ET ARBUSCULE; NODOSITE RACINAIRE; NUDOSIDADES RADICULARES; Phylogeny; Plant Roots; Plants, Medicinal; PROTEINAS; PROTEINE; RACINE; RAICES; RHIZOBIUM LEGUMINOSARUM; SECUENCIA NUCLEOTIDICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Transcription, Genetic; VICIA FABA; Symbiosis; Hemoprotein; Root; Induction; Phycomycetes; Vegetal microorganism relation; Plant nodule; Vicia faba; Gene expression; Rhizobium leguminosarum; Glomus fasciculatum; Fungi; Grain legume; Endomycorrhiza; Leguminosae; Dicotyledones; Angiospermae; Mycorrhiza; Leghemoglobin; Bacteria; Spermatophyta; Rhizobiaceae; Thallophyta
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi.1997.10.1.124

To investigate similarities between symbiotic interactions of broad bean (Vicia faba) with rhizobia and mycorrhizal fungi, plant gene expression induced by both microsymbionts was compared. We demonstrated the exclusive expression of 19 broad bean genes, including VfENOD2, VfENOD5, VfENOD12 and three different leghemoglobin genes, in root nodules. In contrast, the leghemoglobin gene VfLb29 was found to be induced not only in root nodules, but also in broad bean roots colonized by the mycorrhizal fungus Glomus fasciculatum. In uninfected roots, none of the 20 nodulin transcripts investigated was detectable. VfLb29 has an unusually low sequence homology with all other broad bean leghemoglobins as well as with leghemoglobins from other legumes. It can be regarded as a novel kind of leghemoglobin gene not described until now and the induction of which is common to symbiotic interactions of broad bean with both Rhizobium and a mycorrhizal fungus