Characterisation and protein expression profiling of annexins in colorectal cancer

• Published in: British journal of cancer Vol. 98; no. 2; pp. 426 - 433
• Main Authors: Duncan, R, Carpenter, B, Main, L C, Telfer, C, Murray, G I
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.01.2008; Nature Publishing Group
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - mortality; Adenocarcinoma - pathology; Adult; Aged; Aged, 80 and over; Annexins - metabolism; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Breast cancer; Cancer Research; Cell division; Cluster analysis; Colorectal cancer; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - mortality; Colorectal Neoplasms - pathology; Disease Progression; Drug Resistance; Epidemiology; Female; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Male; Medical prognosis; Medical research; Medical sciences; Metastasis; Middle Aged; Molecular Diagnostics; Molecular Medicine; Oncology; Pathology; Prostate cancer; Protein Array Analysis; Protein expression; Proteins; Proteomics; Sarcoma; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Survival Analysis; Tissue Array Analysis; Tumorigenesis; Tumors; United Kingdom > UK; immunohistochemistry; proteomics; tissue microarray; prognosis; annexin; Immunohistochemistry; Rectal disease; Prognosis; Colorectal cancer; Malignant tumor; Protein; Gene expression profile; Colonic disease; Anatomic pathology; Cancerology; Tissue microarrays; Proteomics; Digestive diseases; Intestinal disease; Cancer
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1038/sj.bjc.6604128

The annexins are family of calcium-regulated phospholipid-binding proteins with diverse roles in cell biology. Individual annexins have been implicated in tumour development and progression, and in this investigation a range of annexins have been studied in colorectal cancer. Annexins A1, A2, A4 and A11 were identified by comparative proteomic analysis to be overexpressed in colorectal cancer. Annexins A1, A2, A4 and A11 were further studied by immunohistochemistry with a colorectal cancer tissue microarray containing primary and metastatic colorectal cancer and also normal colon. There was significant increase in expression in annexins A1 ( P =0.01), A2 ( P <0.001), A4 ( P <0.001) and A11 ( P <0.001) in primary tumours compared with normal colon. There was increasing expression of annexins A2 ( P =0.001), A4 ( P =0.03) and A11 ( P =0.006) with increasing tumour stage. An annexin expression profile was identified by k -means cluster analysis, and the annexin profile was associated with tumour stage ( P =0.01) and also patient survival. Patients in annexin cluster group 1 (low annexin expression) had a better survival (log rank=5.33, P =0.02) than patients in cluster group 2 (high annexins A4 and A11 expression). In conclusion, this study has shown that individual annexins are present in colorectal cancer, specific annexins are overexpressed in colorectal cancer and the annexin expression profile is associated with survival.