Quantitative analysis of dopamine receptor messages in the mouse cochlea

• Published in: Brain research. Molecular brain research. Vol. 44; no. 1; pp. 151 - 156
• Main Authors: Karadaghy, Ahmad A, Lasak, John M, Chomchai, Joseph S, Khan, Khalid M, Drescher, Marian J, Drescher, Dennis G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.1997; Elsevier
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cochlea; Cochlea - chemistry; Dopamine; Dopamine receptors; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation; Fundamental and applied biological sciences. Psychology; Mice; Molecular Sequence Data; Neurotransmission; Polymerase Chain Reaction; Quantitative competitive PCR; Receptors, Dopamine - analysis; Vertebrates: nervous system and sense organs; Cochlea; Quantitative competitive PCR; Dopamine; Neurotransmission; Dopamine receptors; Molecular form; Nucleotide sequence; Dopamine receptor; Rodentia; Catecholamine; Inner ear; Organ of hearing; Vertebrata; Mammalia; Mouse; Neurotransmitter; Quantitative analysis
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/S0169-328X(96)00261-6

Dopamine receptor isoforms were examined in the cochlea of the CBA J mouse by RT-PCR analysis and nucleotide sequencing, utilizing primers specific for known dopamine receptor isoforms. Cochlear cDNA sequences corresponding to dopamine D 2(long) and D 3 receptors were amplified, whereas those representing D 1A, D 1B, D 2(short), and D 4 were not detected. Utilizing quantitative competitive PCR analysis, relative levels of dopamine receptor transcripts were found to be 0.002, 0.014, 0.016, and 1.000 for D 2(long) cochlea, D 3 cochlea, D 3 brain, and D 2(long) brain, respectively. In the context of previously published findings, the current work provides key quantitative evidence necessary to establish that dopamine is a neurotransmitter in the auditory inner ear.