HPLC-based quantification of haemagglutinin in the production of egg- and MDCK cell-derived influenza virus seasonal and pandemic vaccines

• Published in: Vaccine Vol. 27; no. 9; pp. 1468 - 1477
• Main Authors: Kapteyn, J.C, Porre, A.M, de Rond, E.J.P, Hessels, W.B, Tijms, M.A, Kessen, H, Slotboom, A.M.E, Oerlemans, M.A, Smit, D, van der Linden, J, Schoen, P, Thus, J.L.G
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 25.02.2009; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Antigens; Applied microbiology; Avian flu; Biological and medical sciences; Cell culture; Chromatography; Chromatography, High Pressure Liquid; Disease Outbreaks - statistics & numerical data; Eggs; Eggs - virology; Fundamental and applied biological sciences. Psychology; H5N1; Haemagglutinin; Hemagglutinins - chemistry; Hemagglutinins - isolation & purification; Humans; Immunization; Influenza vaccine; Influenza Vaccines - immunology; Influenza virus; Influenza, Human - epidemiology; Influenza, Human - immunology; Liquid chromatography; Manufacturing; Methods; Microbiology; Miscellaneous; Netherlands - epidemiology; Pandemics; Reagents; Retention; Seasons; Sensitivity and Specificity; SRID; Studies; Sucrose; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Virology; Netherlands; Solomon Islands; H5N1; Haemagglutinin; SRID; Influenza vaccine; Hemagglutinin; HPLC chromatography; Orthomyxoviridae; Vaccine; Influenzavirus; Infection; Virus; Viral disease; Influenza; Quantitative analysis
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2008.11.113

Abstract The haemagglutinin (HA) content is an important specification of influenza vaccines. Recently, a reversed-phase high performance liquid chromatography (RP-HPLC) method for quantification of HA in PER.C6® cell culture-based whole virus vaccines has been reported, having a high sensitivity, precision, broad range, and high sample throughput [Kapteyn JC, Drissi Saidi M, Dijkstra R, Kars C, Tjon CMS-K, Weverling GJ et al. Haemagglutinin quantification and identification of influenza A&B strains propagated in PER.C6® cells: a novel RP-HPLC method. Vaccine 2006;24:3137–44]. This RP-HPLC assay is based on measuring the peak area of HA1, the hydrophilic subunit of HA, which turned out to be proportional to the amount of HA analyzed. Here, we present data demonstrating that this RP-HPLC method is also highly suitable for HA quantification of active and BPL- or formaldehyde-inactivated egg-based and MDCK cell-based whole virus samples, including egg allantoic harvest, and in final (monovalent) subunit vaccines, including those for pandemic H5N1 strains and for virosomal vaccines. In addition, the RP-HPLC assay was demonstrated to be a very powerful tool in the early stages of seasonal influenza vaccine production, when homologous serial radial immunodiffusion (SRID) reagents are not yet available, enabling fast and reliable viral growth studies in eggs in order to select the best growing virus strains or reassortants for the production of the seasonal trivalent influenza vaccine. Because of its high sensitivity, the RP-HPLC assay has shown its enormous value in supporting small scale MDCK-based (H5N1) influenza virus production models. Finally, the observed differences between HA1 molecules from various HA subtypes in UV absorbance, FLD response, and in the actual retention times in RP-HPLC are discussed in relation to the primary structure of the HA1 molecules studied.