Leukemia inhibitory factor induces cumulus expansion in immature human and mouse oocytes and improves mouse two-cell rate and delivery rates when it is present during mouse in vitro oocyte maturation

• Published in: Fertility and sterility Vol. 90; no. 6; pp. 2367 - 2375
• Main Authors: De Matos, Daniel G., Ph.D, Miller, Kathleen, B.S, Scott, Richard, M.D, Tran, Cam Anh, M.Sc, Kagan, David, M.Sc, Nataraja, Selva G., Ph.D, Clark, Ann, Ph.D, Palmer, Stephen, Ph.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2008; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Proliferation - drug effects; Cleavage Stage, Ovum - drug effects; Cumulus Cells - drug effects; Delivery. Postpartum. Lactation; Dose-Response Relationship, Drug; Embryo Culture Techniques; Embryo Implantation; Embryo Transfer; Female; Fertility Agents, Female - pharmacology; Fertilization in Vitro; Follicle Stimulating Hormone, Human - pharmacology; Gynecology. Andrology. Obstetrics; Humans; Internal Medicine; IVM; Leukemia Inhibitory Factor - pharmacology; LIF; Medical sciences; Mice; Obstetrics and Gynecology; oocytes; Oocytes - drug effects; Pregnancy; Pregnancy Rate; Prospective Studies; Recombinant Proteins - pharmacology; Embryo transfer; LIF; IVM; oocytes; Human; Leukemia inhibitory factor; Gynecology; Rodentia; Assisted procreation; In vitro; Obstetrics; Vertebrata; Mammalia; Mouse; Animal; Delivery; Cell; Oocyte; Oocyte maturation
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/j.fertnstert.2007.10.061

Objective To examine the role of leukemia inhibitory factor (LIF) during in vitro maturation (IVM) on human and mice cumulus expansion and mice oocyte competence by in vitro fertilization (IVF), culture, and embryo transfer (ET). Design Prospective animal and human study. Setting Serono laboratories and IVF clinic. Patient(s) Healthy women volunteers and 8-week-old female mice. Intervention(s) Cumulus compacted human and mice oocytes were matured in IVM media with and without recombinant follicle-stimulating hormone (FSH) and with and without LIF. Mice IVM oocytes with and without 0.2 IU/mL of recombinant FSH; or with and without recombinant FSH + LIF (0.1, 1.0, 1000.0 ng/mL) and ovulated oocytes were in vitro fertilized and cultured. We transferred 395 blastocysts to the uterine horn of 2.5-day pseudopregnant female mice. Main Outcome Measure(s) Cumulus expansion in human and mice oocytes, and two-cell rate, blastocyst rate, and delivered rate of live pups in mice. Result(s) In human and mouse oocytes, LIF induced cumulus expansion. When 1000 ng/mL of LIF was added in combination with recombinant FSH, a statistically significant increase in cleavage rate, embryo development rate, and birth rate was observed when compared with oocytes matured with FSH alone. Conclusion(s) Leukemia inhibitory factor induced cumulus expansion similarly in human and mouse cumulus–oocyte complexes, and recombinant FSH plus LIF supplementation during mouse IVM significantly improved oocyte competence as measured by cleavage rate, blastocyst development, and birth rate.