SIK1-LNC represses the proliferative, migrative, and invasive abilities of lung cancer cells

• Published in: OncoTargets and therapy Vol. 11; pp. 4197 - 4206
• Main Authors: Yang, Liu, Xie, Nianlin, Huang, Jingyu, Huang, Hu, Xu, Shaogan, Wang, Zhigang, Cai, Jun
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2018; Taylor & Francis Ltd; Dove Medical Press
• Subjects: Cancer cells; Cancer metastasis; Cancer research; Cancer therapies; Carcinoma; Cell growth; Chemotherapy; Chromosomes; Gastric cancer; Gene expression; Genomes; Hospitals; Kinases; Lung cancer; Metastasis; Non-small cell lung cancer; Novels; Original Research; Ovarian cancer; Patients; Prostate cancer; Radiation therapy; Surgery; Thoracic surgery; Tumorigenesis; Tumors; United States > US; China; California; lung cancer; SIK1; invasion; proliferation; SIK1-LNC; metastasis
• ISSN: 1178-6930; 1178-6930
• DOI: 10.2147/OTT.S165278

Discussions regarding the correlations between long non-coding RNAs (lncRNAs) and cancers have dominated research in recent years. SIK1-LNC, a type of lncRNA and adjacent to salt-inducible kinases 1 (SIK1), has been found aberrantly expressed in lung cancer. However, its functional role in lung cancer remains largely unknown. In this study, we aimed to explore the association between SIK1-LNC expression and SIK1 in lung cancer cells and further identify the impact of SIK1-LNC on the proliferation, migration invasion of lung cancer cells. Of the 30 patients with non-small-cell lung carcinoma from Zhongnan Hospital of Wuhan University, RT-qPCR was performed to detect SIK1 and SIK1-LNC expressions in patients' samples. Overexpression and knockdown experiments were conducted to analyze the SIK1 and SIK1-LNC expressions in lung cancer cell lines. CCK-8, Brdu, scratch wound-healing, and Transwell assays were respectively employed to evaluate the proliferative, migrative, and invasive abilities of lung cancer cells. Both SIK1-LNC and SIK1 expression levels were evidently downregulated in 30 lung cancer tissues. SIK1-LNC expression was bound up with clinicopathologic features, including lymph node metastasis and distant metastasis. SIK1 expression showed a positive tendency with SIK1-LNC expression in lung cancer cells. SIK1-LNC exerted a significant repression on cell proliferatiive, miogrative and invasive abilities of lung cancer cells. Our findings suggested that SIK1-LNC may act as a novel biomarker and therapeutic target for lung cancer.