Identification of an early subset of cerebellar nuclei neurons in mice

• Published in: eLife Vol. 13
• Main Authors: Rahimi-Balaei, Maryam, Amiri, Shayan, Lamonerie, Thomas, Wu, Sih-Rong, Zoghbi, Huda Y, Consalez, G Giacomo, Goldowitz, Daniel, Marzban, Hassan
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 16.12.2024; eLife Sciences Publications, Ltd; eLife Sciences Publications Ltd
• Subjects: Analysis; Animals; Atoh1; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; cerebellar nuclei; Cerebellar Nuclei - cytology; Cerebellar Nuclei - embryology; Flow Cytometry; Immunohistochemistry; MEIS2; Mice; Mice, Knockout; Neurons; Neurons - cytology; Neurons - physiology; Neuroscience; Otx Transcription Factors - genetics; Otx Transcription Factors - metabolism; OTX2; SNCA; Zoghbi, Huda; United States; Ontario; mouse; cerebellar nuclei; neuroscience; OTX2; MEIS2; Atoh1; SNCA
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.93778

Cerebellar nuclei (CN) neurons serve as the primary output of the cerebellum and originate from the cerebellar primordium at early stages of cerebellar development. These neurons are diverse, integrating information from the cerebellar cortex and relaying it to various brain regions. Employing various methodologies, we have characterized a specific subset of CN neurons that do not originate from the rhombic lip or ventricular zone of the cerebellar primordium. Embryos were collected at early stages of development and processed for immunohistochemistry (IHC), western blotting, in situ hybridization (ISH), embryonic culture, DiI labeling, and flow cytometry analysis (FCM). Our findings indicate that a subset of CN neurons expressing α-synuclein (SNCA), OTX2, MEIS2, and p75NTR (NGFR) are located in the rostroventral region of the NTZ. While CN neurons derived from the rhombic lip are positioned in the caudodorsal area of the NTZ in the cerebellar primordium. Utilizing Otx2-GFP and Atoh1 -/- mice, we have determined that these cells do not originate from the germinal zone of the cerebellar primordium. These results suggest the existence of a novel extrinsic germinal zone for the cerebellar primordium, possibly the mesencephalon, from which early CN neurons originate.