Cloning and characterization of the 5'-transcriptional regulatory region of the human intercellular adhesion molecule 1 gene
Cell-cell adhesion is critical in the generation of immunologic responses and is dependent upon expression of a variety of cell surface receptors. While intercellular adhesion molecule 1 (ICAM-1), a specific receptor for lymphocyte function-associated antigen 1, is constitutively expressed by some c...
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Published in | The Journal of biological chemistry Vol. 266; no. 21; pp. 14024 - 14030 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
25.07.1991
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Subjects | |
Online Access | Get full text |
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Summary: | Cell-cell adhesion is critical in the generation of immunologic responses and is dependent upon expression of a variety of
cell surface receptors. While intercellular adhesion molecule 1 (ICAM-1), a specific receptor for lymphocyte function-associated
antigen 1, is constitutively expressed by some cell types, its de novo or increased expression by various cells has been associated
with the initiation of inflammatory responses and appears to be transcriptionally regulated. The 5' region of the human ICAM-1
gene has been cloned and both structurally and functionally analyzed. A 17.3-kilobase genomic clone containing three exonal
regions encoding the N-terminal third of the ICAM-1 protein was isolated. A 2.05-kilobase subclone, containing the 5' most
exon, was utilized to determine an interferon-gamma-induced transcription initiation site via primer extension and S1 nuclease
protection assays. Analysis of the 5'-flanking region revealed consensus sequences for appropriately located basal promoter
elements, as well as numerous potential cis-acting enhancer elements. When subcloned into a reporter gene construct, the putative
promoter subregion functioned as a potent promoter. However, in accord with biologically observed expression of ICAM-1 in
specific cell types, when additional 5'-flanking sequences were included in reporter gene constructs, tissue appropriate repression
of transcription was observed. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/s0021-9258(18)92805-x |