A new strategy for photoelectrochemical DNA biosensor using chemiluminescence reaction as light source

• Published in: Biosensors & bioelectronics Vol. 26; no. 5; pp. 2737 - 2741
• Main Authors: Zhang, Xiaoru, Zhao, Yanqing, Zhou, Huanran, Qu, Bin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.01.2011; Elsevier
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; Chemiluminescence; Conductometry - instrumentation; detection limit; DNA; DNA - analysis; DNA - genetics; DNA biosensor; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; Lighting - instrumentation; Luminescent Measurements - instrumentation; Methods. Procedures. Technologies; Photoelectrochemistry; Photometry - instrumentation; Ru(bpy) 2dppz 2; Various methods and equipments; Photoelectrochemistry; Chemiluminescence; DNA biosensor; Ru(bpy) 2dppz 2; Photoelectrochemical device; Ru(bpy); Biosensor; DNA; Light; dppz
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2010.09.051

Physical light source is absolutely necessary for usual photoelectrochemical measurement. In this work, chemiluminescence reaction rather than physical light source was used for the development of a novel photoelectrochemical DNA biosensor. CIPO (bis(2,4,5-trichlro-6- n-pentoxycarbonylphenyl)oxalate)–H 2O 2–9,10-diphenylanthrancene was selected as a CL system, which can produce appropriate exciting light and excite photoelectro active materials Ru(bpy) 2dppz 2+ intercalated into the double-stranded DNA. Using such simple intercalation method, a detection limit of 4.5 × 10 −9 M target DNA was achieved without any amplification process. In addition, the selected CL system could be used to excite AuNPs–Ru(bpy) 2dppz 2+ complex as well as CdSe QD multilayer, which indicated a good applicability for the established method.