Heterologous overexpression and preliminary antimicrobial activity test of salmocin M, a novel colicin M-like bacteriocin against Salmonella sp

• Published in: Archives of microbiology Vol. 204; no. 2; p. 154
• Main Authors: Łojewska, Ewelina, Sakowicz, Tomasz, Korycka-Machała, Małgorzata, Kowalczyk, Tomasz
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2022; Springer Nature B.V
• Subjects: Amino acids; Anti-Bacterial Agents - pharmacology; Anti-Infective Agents; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; antimicrobial properties; Bacteria; Bacteriocins; Bacteriocins - genetics; Bacteriocins - pharmacology; Biochemistry; Biological properties; Biomedical and Life Sciences; Biotechnology; Cell Biology; colicins; Colicins - genetics; Colicins - pharmacology; dose response; E coli; Ecology; Escherichia coli; Escherichia coli - genetics; Food industry; Homology; Life Sciences; medicine; Microbial Ecology; Microbiology; Original Paper; protein synthesis; Proteins; recombinant proteins; Salmonella; Salmonella - genetics; Strains (organisms); Bacterial protein production; Antimicrobial activity; Bacteriocins; Recombinant proteins; Salmonella
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-021-02659-y

Currently, it is extremely important to identify and describe new alternative compounds with potential antimicrobial properties. Since various natural biological systems are capable of producing active compounds with such properties, many of them have been the subject of intensive study. The aim of this work was to heterologously overexpress, purify and preliminarily investigate the antimicrobial activity of a novel bacteriocin found in Salmonella species. Overexpressed protein shows an amino acid structure homologous to the well-known colicin M and was never expressed previously in the E. coli platform. Purified salmocin M showed an inhibition spectrum against Salmonella and E. coli strains. To determine its potential as an antimicrobial agent for use in medicine or the food industry, preliminary antimicrobial tests against pathogenic bacteria were carried out. Our research demonstrates that bacteriocin can be produced efficiently in bacterial expression systems, which are one of the cheapest and the most popular platforms for recombinant protein production. Moreover, preliminary results of microbiological tests showed its activity against most of the bacterial strains in a dose-dependent manner. Graphical abstract