A comprehensive and comparative phenotypic analysis of the collaborative founder strains identifies new and known phenotypes

• Published in: Mammalian genome Vol. 31; no. 1-2; pp. 30 - 48
• Main Authors: Kollmus, Heike, Fuchs, Helmut, Lengger, Christoph, Haselimashhadi, Hamed, Bogue, Molly A., Östereicher, Manuela A., Horsch, Marion, Adler, Thure, Aguilar-Pimentel, Juan Antonio, Amarie, Oana Veronica, Becker, Lore, Beckers, Johannes, Calzada-Wack, Julia, Garrett, Lillian, Hans, Wolfgang, Hölter, Sabine M., Klein-Rodewald, Tanja, Maier, Holger, Mayer-Kuckuk, Philipp, Miller, Gregor, Moreth, Kristin, Neff, Frauke, Rathkolb, Birgit, Rácz, Ildikó, Rozman, Jan, Spielmann, Nadine, Treise, Irina, Busch, Dirk, Graw, Jochen, Klopstock, Thomas, Wolf, Eckhard, Wurst, Wolfgang, Yildirim, Ali Önder, Mason, Jeremy, Torres, Arturo, Balling, Rudi, Mehaan, Terry, Gailus-Durner, Valerie, Schughart, Klaus, Hrabě de Angelis, Martin
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2020; Springer Nature B.V
• Subjects: Allergies; Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; blood chemistry; Cartilage; Cell Biology; Collaboration; Collaborative Cross Mice - genetics; Databases, Genetic; Energy metabolism; eyes; Female; Genetic Association Studies; genome; Genotype; Genotypes; Human Genetics; humans; hypersensitivity; immunology; Inbreeding; Life Sciences; lung function; Male; Mice; Mice, Inbred Strains - genetics; nociception; Pain perception; Phenotype; Phenotypes; Phenotyping; Quantitative Trait Loci; Respiratory function; Species Specificity; Strains (organisms); vision
• ISSN: 0938-8990; 1432-1777; 1432-1777
• DOI: 10.1007/s00335-020-09827-3

The collaborative cross (CC) is a large panel of mouse-inbred lines derived from eight founder strains (NOD/ShiLtJ, NZO/HILtJ, A/J, C57BL/6J, 129S1/SvImJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ). Here, we performed a comprehensive and comparative phenotyping screening to identify phenotypic differences and similarities between the eight founder strains. In total, more than 300 parameters including allergy, behavior, cardiovascular, clinical blood chemistry, dysmorphology, bone and cartilage, energy metabolism, eye and vision, immunology, lung function, neurology, nociception, and pathology were analyzed; in most traits from sixteen females and sixteen males. We identified over 270 parameters that were significantly different between strains. This study highlights the value of the founder and CC strains for phenotype-genotype associations of many genetic traits that are highly relevant to human diseases. All data described here are publicly available from the mouse phenome database for analyses and downloads.