preliminary linkage map of the hard tick, Ixodes scapularis

• Published in: Insect molecular biology Vol. 12; no. 2; pp. 201 - 210
• Main Authors: Ullmann, A.J, Piesman, J, Dolan, M.C, Black, W.C. IV
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.04.2003
• Subjects: Animals; cDNA-SSCP; chromosome mapping; Chromosome Mapping - methods; complementary DNA; Crosses, Genetic; crossing; Female; Gene Library; Genes, Insect - genetics; Genetic Markers; genetic techniques and protocols; Genome; Ixodes - genetics; Ixodes scapularis; linkage (genetics); linkage groups; linkage map; loci; Male; Microsatellite Repeats; microsatellites; polymerase chain reaction; Polymorphism, Single-Stranded Conformational; Random Amplified Polymorphic DNA Technique; RAPD-SSCP; sequence tagged random amplified polymorphic DNA markers; Sequence Tagged Sites; single strand conformation polymorphism; STARs; ticks
• ISSN: 0962-1075; 1365-2583
• DOI: 10.1046/j.1365-2583.2003.00402.x

A linkage map of the Ixodes scapularis genome was constructed, based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence‐Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field‐collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ≈ 109 bp, the relationship of physical to genetic distance was found to be ≈ 300 kb/cM in the I. scapularis genome.