Novel methods for the detection of microbial antibodies in oral fluid

Compared with blood, oral fluid has several advantages as a sample for antibody detection. It is simple, safe, painless, and cheap to collect. The only drawback is that while the antibody profiles indicate those in blood, they are at lower concentrations. Antibody capture assays are the method of ch...

Full description

Saved in:
Bibliographic Details
Published inThe Lancet infectious diseases Vol. 2; no. 1; pp. 18 - 24
Main Authors McKie, Anne, Vyse, Andrew, Maple, Christopher
Format Journal Article
LanguageEnglish
Published United States Elsevier Ltd 2002
Elsevier Limited
Subjects
Antibodies - isolation & purification
Enzyme-Linked Immunosorbent Assay - methods
Fluoroimmunoassay - methods
Humans
Immunoassay - methods
Infectious diseases
Polymerase Chain Reaction
Radioimmunoassay - methods
Saliva - immunology
Specimen Handling - methods
Online AccessGet full text

Cover

More Information
Summary:Compared with blood, oral fluid has several advantages as a sample for antibody detection. It is simple, safe, painless, and cheap to collect. The only drawback is that while the antibody profiles indicate those in blood, they are at lower concentrations. Antibody capture assays are the method of choice for the detection of microbial antibodies in oral fluid, but their relative lack of sensitivity when based on conventional immunoassay techniques has mostly limited their use to epidemiological applications. Immuno-PCR and time-resolved fluorescence offer more sensitive detection systems that could be applied to oral fluid specimens. We review antibody detection in oral fluid and discuss immuno-PCR and time-resolved fluorescence as candidate systems. Both have the potential to broaden the applications of oral fluid testing to clinical diagnostics.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:1473-3099
1474-4457
DOI:10.1016/S1473-3099(01)00169-4