Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1814; p. 63
• Main Authors: Maity, Sibaprasad, Viazovkina, Ekaterina, Gall, Alexander, Lyubchenko, Yuri L
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Nanoprobing; AFM; Click chemistry; Force spectroscopy; Protein-protein interactions; Amyloid oligomers
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-8591-3_5

Pair-wise interactions at the single-molecule level can be done with nanoprobing techniques, such as AFM force spectroscopy, optical tweezers, and magnetic tweezers. These techniques can be used to probe interactions between well-characterized assemblies of biomolecules, such as monomer-dimer, dimer-dimer, and trimer-monomer. An important step of these techniques is the proper assembly of dimers, trimers, and higher oligomers to enable the interactions to be probed. We have developed a novel approach in which a defined number of peptides are assembled along a flexible polymeric molecule that serves as a linear matrix, termed as flexible nanoarray (FNA). The construct is synthesized with the use of phosphoramidite chemistry (PA), in which non-nucleoside PA spacers and standard oligonucleotide synthesis are used to grow the polymeric chain with the desired length. The reactive sites are incorporated during FNA synthesis. As a result, the FNA polymer contains a set of predesigned reactive sites to which the peptides are covalently conjugated. We describe the protocol for the synthesis of FNA and the application of this methodology to measure the molecular interactions between amyloid peptides of monomer-monomer, monomer-dimer, and dimer-dimer.