Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection

Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or...

Full description

Saved in:
Bibliographic Details
Published inMicroorganisms (Basel) Vol. 9; no. 4; p. 674
Main Authors Leon, Fanny, Pinchon, Elena, Temurok, Nevzat, Morvan, François, Vasseur, Jean-Jacques, Clot, Martine, Foulongne, Vincent, Cantaloube, Jean-François, Vande Perre, Philippe, Molès, Jean-Pierre, Daynès, Aurélien, Fournier-Wirth, Chantal
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 24.03.2021
MDPI
Subjects
Agglutination
Antibodies
Antigens
arbovirus
Biotechnology
Biotin
Blood & organ donations
Brief Report
Dengue fever
DNA probes
Enzyme-linked immunosorbent assay
Epidemiology
Genomes
Human health and pathology
Immunology
Infections
Infectious diseases
innovative diagnostic
Life Sciences
magnetic agglutination
Magnetic fields
Microbiology and Parasitology
Nanoparticles
Plasma
Polyethylene glycol
Polymerase chain reaction
Sensitivity
Serology
Signal processing
Surfactants
Turbidimetry
Vaccines
Vector-borne diseases
viral genomes
Virology
Viruses
West Nile virus
Zika virus
France
United States > US
Germany
viral genomes
antibodies
innovative diagnostic
nanoparticles
arbovirus
magnetic agglutination
Nanoparticles
Innovative diagnostic
Viral genomes
Antibodies
Arbovirus
Magnetic agglutination
Online AccessGet full text

Cover

Abstract Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(-) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%-97.95%) and 96.87% (95% CI, 90.84%-100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%-97.76%) and 97.44% (95% CI, 92.48%-100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections.
AbstractList Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections
Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections.
Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(-) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%-97.95%) and 96.87% (95% CI, 90.84%-100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%-97.76%) and 97.44% (95% CI, 92.48%-100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections.
Author Clot, Martine
Leon, Fanny
Vande Perre, Philippe
Pinchon, Elena
Morvan, François
Temurok, Nevzat
Molès, Jean-Pierre
Daynès, Aurélien
Fournier-Wirth, Chantal
Cantaloube, Jean-François
Vasseur, Jean-Jacques
Foulongne, Vincent
AuthorAffiliation 1 Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France; fanny.leon@efs.sante.fr (F.L.); elena.pinchon@efs.sante.fr (E.P.); v-foulongne@chu-montpellier.fr (V.F.); jean-francois.cantaloube@efs.sante.fr (J.-F.C.); p-van_de_perre@chu-montpellier.fr (P.V.P.); jean-pierre.moles@inserm.fr (J.-P.M.)
3 Institut des Biomolecules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, France; francois.morvan@umontpellier.fr (F.M.); jean-jacques.vasseur@umontpellier.fr (J.-J.V.)
2 HORIBA Medical, 34184 Montpellier, France; nevzat.temurok@horiba.com (N.T.); martine.clot@horiba.com (M.C.); aurelien.daynes@horiba.com (A.D.)
AuthorAffiliation_xml – name: 1 Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France; fanny.leon@efs.sante.fr (F.L.); elena.pinchon@efs.sante.fr (E.P.); v-foulongne@chu-montpellier.fr (V.F.); jean-francois.cantaloube@efs.sante.fr (J.-F.C.); p-van_de_perre@chu-montpellier.fr (P.V.P.); jean-pierre.moles@inserm.fr (J.-P.M.)
– name: 3 Institut des Biomolecules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, France; francois.morvan@umontpellier.fr (F.M.); jean-jacques.vasseur@umontpellier.fr (J.-J.V.)
– name: 2 HORIBA Medical, 34184 Montpellier, France; nevzat.temurok@horiba.com (N.T.); martine.clot@horiba.com (M.C.); aurelien.daynes@horiba.com (A.D.)
Author_xml – sequence: 1
  givenname: Fanny
  surname: Leon
  fullname: Leon, Fanny
  organization: Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
– sequence: 2
  givenname: Elena
  surname: Pinchon
  fullname: Pinchon, Elena
  organization: Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
– sequence: 3
  givenname: Nevzat
  surname: Temurok
  fullname: Temurok, Nevzat
  organization: HORIBA Medical, 34184 Montpellier, France
– sequence: 4
  givenname: François
  orcidid: 0000-0001-5077-0028
  surname: Morvan
  fullname: Morvan, François
  organization: Institut des Biomolecules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, France
– sequence: 5
  givenname: Jean-Jacques
  orcidid: 0000-0002-4379-6139
  surname: Vasseur
  fullname: Vasseur, Jean-Jacques
  organization: Institut des Biomolecules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, France
– sequence: 6
  givenname: Martine
  surname: Clot
  fullname: Clot, Martine
  organization: HORIBA Medical, 34184 Montpellier, France
– sequence: 7
  givenname: Vincent
  orcidid: 0000-0003-3741-754X
  surname: Foulongne
  fullname: Foulongne, Vincent
  organization: Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
– sequence: 8
  givenname: Jean-François
  surname: Cantaloube
  fullname: Cantaloube, Jean-François
  organization: Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
– sequence: 9
  givenname: Philippe
  surname: Vande Perre
  fullname: Vande Perre, Philippe
  organization: Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
– sequence: 10
  givenname: Jean-Pierre
  orcidid: 0000-0002-6863-6350
  surname: Molès
  fullname: Molès, Jean-Pierre
  organization: Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
– sequence: 11
  givenname: Aurélien
  orcidid: 0000-0001-7921-4164
  surname: Daynès
  fullname: Daynès, Aurélien
  organization: HORIBA Medical, 34184 Montpellier, France
– sequence: 12
  givenname: Chantal
  orcidid: 0000-0001-6118-2918
  surname: Fournier-Wirth
  fullname: Fournier-Wirth, Chantal
  organization: Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, France
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33805173$$D View this record in MEDLINE/PubMed
https://hal.umontpellier.fr/hal-03198157$$DView record in HAL
BookMark eNptktFqFDEUhgep2Fr7CErAq16MJpNkMrkRlnbbXVhRRL0NmeTMbJadpCYzCz6I72vGraUVc5Nw_vzf4Rz-l8WJDx6K4jXB7yiV-P3gTAwh9tq7NCSJGa4Fe1acVVjUZVVjcfLofVpcpLTD-UhCG05eFKeUNpgTQc-KX9dO9z6k0Rn0GWIX4qC9ARQ6pNHHLMGs3DjY23Lpt7Nm0aLv99PovB5d8OgLaBumETmPrmEEk4UeLd24hYi-u6j36BZ8GCChENEqt0ILP7o2WJdL2bSIbTi4OCW09t1sD_5V8bzT-wQX9_d58e1m-fVqVW4-3a6vFpvScFyPZUfaPASzjFRNrTEQVlNWdaY2loFhgCsucWc72jIBUgJvCecghdCEysoael6sj1wb9E7dRTfo-FMF7dSfQt6w0jEvYA8KrGCYNMYYahkHJmUleMW7BmSNGW0y68ORdTe1A1gDfsyzP4E-Vbzbqj4cVIPr7J8Bl0fA9h_barFRcw1TIhvCxYHkv2_vm8XwY4I0ql2Yos-7UhWnmNSsEjORH3_ltKQUoXvAEqzmIKn_Bin73jwe5cH1Nzb0Nx6fy4Q
CitedBy_id crossref_primary_10_1016_j_bios_2022_114560
crossref_primary_10_3389_fchem_2021_817246
Cites_doi 10.1021/acssensors.0c01488
10.1016/j.jmoldx.2018.08.005
10.1021/acsnano.5b02379
10.1016/j.cca.2019.03.008
10.1016/j.jcv.2018.08.007
10.20944/preprints201908.0057.v1
10.3390/s16060828
10.1146/annurev-med-050715-105122
10.1016/j.jmoldx.2020.06.012
10.1038/nrmicro2459
10.3390/diagnostics10050302
10.1002/adma.201904385
10.1016/S1473-3099(18)30269-X
10.1128/JCM.39.5.1922-1927.2001
10.1126/science.abb6143
10.1039/C8LC01323C
10.1016/j.cmi.2017.10.035
10.1371/journal.pntd.0008082
10.3390/microorganisms8010084
10.1002/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E
10.1038/s41577-020-00410-0
10.1038/nrmicro2460
10.1371/journal.pntd.0007314
10.1093/infdis/jix678
10.1038/nrdp.2016.55
10.1016/S1473-3099(16)30473-X
10.1021/nl200384p
10.1038/nrmicro1522z
10.1016/j.talanta.2020.121344
ContentType Journal Article
Copyright 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Attribution
2021 by the authors. 2021
Copyright_xml – notice: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: Attribution
– notice: 2021 by the authors. 2021
DBID NPM
AAYXX
CITATION
7T7
8FD
8FE
8FH
ABUWG
AFKRA
ATCPS
AZQEC
BBNVY
BENPR
BHPHI
C1K
CCPQU
DWQXO
FR3
GNUQQ
HCIFZ
LK8
M7P
P64
PATMY
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
PYCSY
1XC
VOOES
5PM
DOA
DOI 10.3390/microorganisms9040674
DatabaseName PubMed
CrossRef
Industrial and Applied Microbiology Abstracts (Microbiology A)
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Agriculture & Environmental Science Database
ProQuest Central Essentials
Biological Science Collection
AUTh Library subscriptions: ProQuest Central
ProQuest Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One Community College
ProQuest Central
Engineering Research Database
ProQuest Central Student
SciTech Premium Collection (Proquest) (PQ_SDU_P3)
Biological Sciences
Biological Science Database
Biotechnology and BioEngineering Abstracts
Environmental Science Database
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
Environmental Science Collection
Hyper Article en Ligne (HAL)
Hyper Article en Ligne (HAL) (Open Access)
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle PubMed
CrossRef
Publicly Available Content Database
ProQuest Central Student
Technology Research Database
ProQuest Central Essentials
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest Central
Natural Science Collection
ProQuest Central Korea
Agricultural & Environmental Science Collection
Biological Science Collection
Industrial and Applied Microbiology Abstracts (Microbiology A)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Biological Science Database
ProQuest SciTech Collection
Biotechnology and BioEngineering Abstracts
Environmental Science Collection
ProQuest One Academic UKI Edition
Environmental Science Database
Engineering Research Database
ProQuest One Academic
DatabaseTitleList

Publicly Available Content Database

PubMed
CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: AUTh Library subscriptions: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2076-2607
ExternalDocumentID oai_doaj_org_article_ed74018ccc3d45e49927525f8e960438
oai_HAL_hal_03198157v1
10_3390_microorganisms9040674
33805173
Genre Journal Article
GeographicLocations France
United States--US
Germany
GeographicLocations_xml – name: France
– name: United States--US
– name: Germany
GrantInformation_xml – fundername: French Blood Establishment
  grantid: ArboMag project
– fundername: HORIBA Medical, Montpellier France
  grantid: ArboMag project
– fundername: Inserm
  grantid: ArboMag project
– fundername: University of Montpellier, I-SITE MUSE
  grantid: ArboMag project
GroupedDBID 53G
5VS
7XC
8FE
8FH
AADQD
AAFWJ
AAHBH
ACPRK
AFKRA
AFPKN
AFRAH
AFZYC
ALMA_UNASSIGNED_HOLDINGS
ATCPS
BBNVY
BENPR
BHPHI
CCPQU
ECGQY
GROUPED_DOAJ
GS5
GX1
HCIFZ
HYE
IAO
KQ8
LK8
M48
M7P
MODMG
M~E
NPM
OK1
PATMY
PGMZT
PIMPY
PROAC
PYCSY
RIG
RNS
RPM
AAYXX
CITATION
7T7
8FD
ABUWG
AZQEC
C1K
DWQXO
FR3
GNUQQ
P64
PQEST
PQQKQ
PQUKI
PRINS
1XC
ADRAZ
IPNFZ
ITC
VOOES
5PM
ID FETCH-LOGICAL-c506t-f1b1734d41286a0e146342fc6cd4ec4e02590fdf3b47e99e5b155e977a1392dc3
IEDL.DBID RPM
ISSN 2076-2607
IngestDate Tue Oct 22 15:16:28 EDT 2024
Tue Sep 17 21:33:18 EDT 2024
Tue Oct 15 16:04:26 EDT 2024
Thu Oct 10 18:37:09 EDT 2024
Fri Aug 23 04:41:39 EDT 2024
Wed Oct 16 00:42:28 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords viral genomes
antibodies
innovative diagnostic
nanoparticles
arbovirus
magnetic agglutination
Nanoparticles
Innovative diagnostic
Viral genomes
Antibodies
Arbovirus
Magnetic agglutination
Language English
License Attribution: http://creativecommons.org/licenses/by
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c506t-f1b1734d41286a0e146342fc6cd4ec4e02590fdf3b47e99e5b155e977a1392dc3
Notes PMCID: PMC8064388
Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Site EFS TransDiag, 240 avenue du Pr J.L Viala, CEDEX 4, 34184 Montpellier, France.
ORCID 0000-0002-4379-6139
0000-0001-6118-2918
0000-0002-6863-6350
0000-0003-3741-754X
0000-0001-5077-0028
0000-0001-7921-4164
0000-0001-6275-1668
0000-0002-3912-0427
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064388/
PMID 33805173
PQID 2530164278
PQPubID 2032358
ParticipantIDs doaj_primary_oai_doaj_org_article_ed74018ccc3d45e49927525f8e960438
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8064388
hal_primary_oai_HAL_hal_03198157v1
proquest_journals_2530164278
crossref_primary_10_3390_microorganisms9040674
pubmed_primary_33805173
PublicationCentury 2000
PublicationDate 20210324
PublicationDateYYYYMMDD 2021-03-24
PublicationDate_xml – month: 3
  year: 2021
  text: 20210324
  day: 24
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Microorganisms (Basel)
PublicationTitleAlternate Microorganisms
PublicationYear 2021
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Guzman (ref_4) 2016; 2
Mezger (ref_29) 2015; 9
Moerland (ref_30) 2019; 19
ref_13
Bournazos (ref_19) 2020; 20
Ranzoni (ref_25) 2011; 11
Chen (ref_8) 2019; 493
Weaver (ref_2) 2018; 69
Peeling (ref_7) 2010; 8
Pinchon (ref_12) 2020; 219
Goncalves (ref_9) 2018; 217
Newcombe (ref_15) 1998; 17
Leon (ref_11) 2019; 21
Gloag (ref_26) 2019; 31
Guzman (ref_10) 2010; 8
Petersen (ref_18) 2018; 24
Peeling (ref_23) 2010; 8
ref_21
ref_3
ref_28
Scaramozzino (ref_14) 2001; 39
Bezinge (ref_24) 2020; 5
ref_27
Katzelnick (ref_20) 2020; 369
Musso (ref_1) 2018; 18
Barzon (ref_17) 2018; 107
Katzelnick (ref_5) 2017; 17
Armbruster (ref_16) 2008; 29
Morjaria (ref_22) 2020; 22
ref_6
References_xml – volume: 5
  start-page: 2701
  year: 2020
  ident: ref_24
  article-title: Enzyme-Assisted Nucleic Acid Detection for Infectious Disease Diagnostics: Moving toward the Point-of-Care
  publication-title: ACS Sens
  doi: 10.1021/acssensors.0c01488
  contributor:
    fullname: Bezinge
– volume: 21
  start-page: 81
  year: 2019
  ident: ref_11
  article-title: An Innovative Multiplexed and Flexible Molecular Approach for the Differential Detection of Arboviruses
  publication-title: J. Mol. Diagn.
  doi: 10.1016/j.jmoldx.2018.08.005
  contributor:
    fullname: Leon
– volume: 9
  start-page: 7374
  year: 2015
  ident: ref_29
  article-title: Scalable DNA-Based Magnetic Nanoparticle Agglutination Assay for Bacterial Detection in Patient Samples
  publication-title: ACS Nano
  doi: 10.1021/acsnano.5b02379
  contributor:
    fullname: Mezger
– volume: 493
  start-page: 138
  year: 2019
  ident: ref_8
  article-title: Point of care testing for infectious diseases
  publication-title: Clin. Chim. Acta
  doi: 10.1016/j.cca.2019.03.008
  contributor:
    fullname: Chen
– volume: 107
  start-page: 38
  year: 2018
  ident: ref_17
  article-title: Ongoing and emerging arbovirus threats in Europe
  publication-title: J. Clin. Virol.
  doi: 10.1016/j.jcv.2018.08.007
  contributor:
    fullname: Barzon
– ident: ref_21
  doi: 10.20944/preprints201908.0057.v1
– ident: ref_27
  doi: 10.3390/s16060828
– volume: 69
  start-page: 395
  year: 2018
  ident: ref_2
  article-title: Zika, Chikungunya, and Other Emerging Vector-Borne Viral Diseases
  publication-title: Annu. Rev. Med.
  doi: 10.1146/annurev-med-050715-105122
  contributor:
    fullname: Weaver
– volume: 22
  start-page: 1109
  year: 2020
  ident: ref_22
  article-title: Who to Test, When, and for What: Why Diagnostic Stewardship in Infectious Diseases Matters
  publication-title: J. Mol. Diagn.
  doi: 10.1016/j.jmoldx.2020.06.012
  contributor:
    fullname: Morjaria
– volume: 8
  start-page: S30
  year: 2010
  ident: ref_7
  article-title: Evaluation of diagnostic tests: Dengue
  publication-title: Nat. Rev. Microbiol.
  doi: 10.1038/nrmicro2459
  contributor:
    fullname: Peeling
– volume: 29
  start-page: S49
  year: 2008
  ident: ref_16
  article-title: Limit of blank, limit of detection and limit of quantitation
  publication-title: Clin. Biochem. Rev.
  contributor:
    fullname: Armbruster
– ident: ref_13
  doi: 10.3390/diagnostics10050302
– volume: 31
  start-page: e1904385
  year: 2019
  ident: ref_26
  article-title: Advances in the Application of Magnetic Nanoparticles for Sensing
  publication-title: Adv. Mater.
  doi: 10.1002/adma.201904385
  contributor:
    fullname: Gloag
– volume: 18
  start-page: e355
  year: 2018
  ident: ref_1
  article-title: Unexpected outbreaks of arbovirus infections: Lessons learned from the Pacific and tropical America
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(18)30269-X
  contributor:
    fullname: Musso
– volume: 39
  start-page: 1922
  year: 2001
  ident: ref_14
  article-title: Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.39.5.1922-1927.2001
  contributor:
    fullname: Scaramozzino
– volume: 369
  start-page: 1123
  year: 2020
  ident: ref_20
  article-title: Zika virus infection enhances future risk of severe dengue disease
  publication-title: Science
  doi: 10.1126/science.abb6143
  contributor:
    fullname: Katzelnick
– volume: 19
  start-page: 919
  year: 2019
  ident: ref_30
  article-title: Rotating magnetic particles for lab-on-chip applications—A comprehensive review
  publication-title: Lab Chip
  doi: 10.1039/C8LC01323C
  contributor:
    fullname: Moerland
– volume: 24
  start-page: 369
  year: 2018
  ident: ref_18
  article-title: Emerging infections-an increasingly important topic: Review by the Emerging Infections Task Force
  publication-title: Clin. Microbiol. Infect.
  doi: 10.1016/j.cmi.2017.10.035
  contributor:
    fullname: Petersen
– ident: ref_28
  doi: 10.1371/journal.pntd.0008082
– ident: ref_3
  doi: 10.3390/microorganisms8010084
– volume: 17
  start-page: 857
  year: 1998
  ident: ref_15
  article-title: Two-sided confidence intervals for the single proportion: Comparison of seven methods
  publication-title: Stat. Med.
  doi: 10.1002/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E
  contributor:
    fullname: Newcombe
– volume: 20
  start-page: 633
  year: 2020
  ident: ref_19
  article-title: The role of IgG Fc receptors in antibody-dependent enhancement
  publication-title: Nat. Rev. Immunol.
  doi: 10.1038/s41577-020-00410-0
  contributor:
    fullname: Bournazos
– volume: 8
  start-page: S7
  year: 2010
  ident: ref_10
  article-title: Dengue: A continuing global threat
  publication-title: Nat. Rev. Microbiol.
  doi: 10.1038/nrmicro2460
  contributor:
    fullname: Guzman
– ident: ref_6
  doi: 10.1371/journal.pntd.0007314
– volume: 217
  start-page: 1060
  year: 2018
  ident: ref_9
  article-title: Innovative and New Approaches to Laboratory Diagnosis of Zika and Dengue: A Meeting Report
  publication-title: J. Infect. Dis.
  doi: 10.1093/infdis/jix678
  contributor:
    fullname: Goncalves
– volume: 2
  start-page: 16055
  year: 2016
  ident: ref_4
  article-title: Dengue infection
  publication-title: Nat. Rev. Dis. Primers
  doi: 10.1038/nrdp.2016.55
  contributor:
    fullname: Guzman
– volume: 17
  start-page: e88
  year: 2017
  ident: ref_5
  article-title: Dengue: Knowledge gaps, unmet needs, and research priorities
  publication-title: Lancet Infect. Dis
  doi: 10.1016/S1473-3099(16)30473-X
  contributor:
    fullname: Katzelnick
– volume: 11
  start-page: 2017
  year: 2011
  ident: ref_25
  article-title: Frequency-selective rotation of two-particle nanoactuators for rapid and sensitive detection of biomolecules
  publication-title: Nano Lett.
  doi: 10.1021/nl200384p
  contributor:
    fullname: Ranzoni
– volume: 8
  start-page: S2
  year: 2010
  ident: ref_23
  article-title: A guide for diagnostic evaluations
  publication-title: Nat. Rev. Microbiol.
  doi: 10.1038/nrmicro1522z
  contributor:
    fullname: Peeling
– volume: 219
  start-page: 121344
  year: 2020
  ident: ref_12
  article-title: Rapid and specific DNA detection by magnetic-field enhanced agglutination assay
  publication-title: Talanta
  doi: 10.1016/j.talanta.2020.121344
  contributor:
    fullname: Pinchon
SSID ssj0000913851
Score 2.2158604
Snippet Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and...
SourceID doaj
pubmedcentral
hal
proquest
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 674
SubjectTerms Agglutination
Antibodies
Antigens
arbovirus
Biotechnology
Biotin
Blood & organ donations
Brief Report
Dengue fever
DNA probes
Enzyme-linked immunosorbent assay
Epidemiology
Genomes
Human health and pathology
Immunology
Infections
Infectious diseases
innovative diagnostic
Life Sciences
magnetic agglutination
Magnetic fields
Microbiology and Parasitology
Nanoparticles
Plasma
Polyethylene glycol
Polymerase chain reaction
Sensitivity
Serology
Signal processing
Surfactants
Turbidimetry
Vaccines
Vector-borne diseases
viral genomes
Virology
Viruses
West Nile virus
Zika virus
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9QwELVQpUpcEJSvlLayENfQJGMn8THQXRZEEQeKeosSx-7msAlKspX4If2_nYnTsgEkLlxysB3HyYw9z_GbGcbe2CLQFmLiVIHwBRTKV2hH_DCxAUQ6NCmQg_P5l3h1IT5dysudVF_ECXPhgd2HOzUV5YxLtdZQCWkQoEeJjKRNDYUVAefmG6idzdS4BqsQEEs4lx3Aff3phvhtLlNSv-kV6m6ciJkxGmP2o4lZEyPyT7j5O2tyxwwtH7NHE37kmRv3E_bANAds32WU_PmU3Zw56hzW8q-_fAJ4a3nBz7GKfBb5kmhr_qJZj8f_PLu6Iv1z_wU50erb7cDrhp8ZOmJA48YX5LrR8e81Dol_ME27MT1vO77CR_GsGeqyJToi3ZR1ZXtdd9uef5yIXs0zdrFcfHu_8qfMC76WQTz4NizDBEQl0HrFRWBwOQURWR3rShgtDAIlFdjKQikSo5SRJcISg1CyQEAZVRqes72mbcxLxqWKK7AgdVwWAvASg9ZpqCUiSVUq67G3dyLIf7gAGzluTEhm-V9l5rF3JKj7xhQfeyzAhvmkNfm_tMZjr1HMsz5W2eecysitKw1lch167OhOC_JpZvd5JIGikkUJ9vHCKcR9N7jdp5Bn4LFkpiqz58xrmno9xvROCRqm6eH_eLlX7GFEzJsA_Egcsb2h25pjhE5DeTLOklt77Rkt
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: AUTh Library subscriptions: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELZgKyQuiDeBgizENTTxI7FPVUp3WRCtKkRRb1Hi2N0cNilJtlJ_CP-XmSS7JYC45GA7caQZe76xv5kh5J3LAuN4hJwqLnzBM-1rsCN-GLuAMxNaxTHA-eQ0Wp6LzxfyYjxwa0da5XZP7DfqojZ4Rn7AJMdsUCxWh1c_fKwahberYwmNu2SPgafAZmTvaH569nV3yoJZLwFTDKE7HPz7gzXy3IaKSe261aDDUSwmRqnP3Q-mZoXMyL9h55_syd_M0eIheTDiSJoMgn9E7tjqMbk3VJa8eUJ-Hg8UOuilZ7exAbR2NKMn0IWxi3SB9DV_Xq16GgBNLi9RD4fzQYr0-nrT0bKixxavGsDI0TmGcDT0ewm_RD_aql7bltYNXcJUNKm6Mq-RlogvJU1eX5fNpqWfRsJX9ZScL-bfPiz9sQKDb2QQdb4L8zDmohBgxaIssLCtcsGciUwhrBEWAJMOXOF4LmKrtZU5wBMLkDIDYMkKw5-RWVVX9gWhUkcFd1yaKM8Eh0fEjVGhkYAoda6dR95vRZBeDYk2UnBQUGbpP2XmkSMU1G4w5snuG2BgOi671BZYcVAZY3ghpAX3jsWSSacsJqXhyiNvQcyTbyyTLym2YXiXCmV8HXpkf6sF6bjC2_RWHz3yfFCI3WfA7cfUZ9wj8URVJvNMe6py1ef2VggRlXr5_ylfkfsMuTUB95nYJ7Ou2djXAI66_M24An4BTeQS3Q
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Journals: Open Access(OpenAccess)
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lj5RAEO6sazR7Mb5lXU3HeGWFfkD3wRh0ZxyNazw4Zm8Emu4dEgdcYDbuD_H_WsVjdXRNvHCgoSFUNfUVfPUVIc9dFhjHI-RUceELnmlfQxzxw9gFnJnQKo4Fzscfo8VSvD-RJztkElQYH2B7ZWqH_aSWzdfD72cXr2DBv8SME1L2F2ukrg1NkNp1q8Eto1hcI9cZanMhm29E_P3LWYdc9U0ZGWTwPqD5eKjr-fdMe-Qm5HGoZcW3glev8Q8haYUMyr_h6Z8sy9_C1vw2uTXiTZoMDnKH7NjqLrkxdKC8uEd-HA1UOxiln37VENDa0YwewxDWONI50tz8WbXq6QI0OT1Ffx2-I1Kk4debjpYVPbL4SwKCIZ1hqUdDv5RwS_Streq1bWnd0AVciiZVV-Y10hfxpKTJ6_Oy2bT03UgMq-6T5Xz2-c3CHzs1-EYGUee7MIenIwoB0S7KAguvXy6YM5EphDXCArDSgSscz0VstbYyBxhjAXpmAEBZYfgDslvVlX1EqNRRwR2XJsozwWETcWNUaCQgT51r55HDyQTpt0GQI4VEBs2XXmk-j7xGQ10ejHra_Q44MB2XZ2oL7EyojDG8ENJCGshiyaRTFsVruPLIMzDz1hyL5EOK-7AMTIUyPg89cjB5QTo5csokRxUzFsMcDweHuJxmciuPxFuusnWd7ZGqXPUa4AqhpFL7_3Nfj8keQyZOwH0mDshu12zsE4BSXf60Xxw_AXJLHcE
  priority: 102
  providerName: Scholars Portal
Title Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
URI https://www.ncbi.nlm.nih.gov/pubmed/33805173
https://www.proquest.com/docview/2530164278
https://hal.umontpellier.fr/hal-03198157
https://pubmed.ncbi.nlm.nih.gov/PMC8064388
https://doaj.org/article/ed74018ccc3d45e49927525f8e960438
Volume 9
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb5wwELWSVJV6qfod2nRlVb2yC9gGfCTJbrdVN1pVTZUbAmNnkQpEwEbqD-n_7QwfaWh76oUDBoOYB_PGvJkh5L1JHGWYj5oqxm3OEmlL8CO2GxiHecrVIcME582Fv77kn67E1QERYy5MJ9pXaT4vvxfzMt912sqbQi1GndhiuzkL0Y9C5HZIDgGg90L07vMrXQY0os_WYRDSLwqUtvVNkpqikQBbP8B-PBCdYYUqNnFJXeV-cDQ71EX-TTr_1E7ec0arJ-TxwCJp1N_tU3Kgy2fkYd9X8sdz8vO8F9DBKN3-zgyglaEJ3cAQZi7SFYrX7GW560QANLq-RhT2q4MUxfXVvqV5Sc81_mgAF0eXmMBR02853BL9oMuq0A2tarqGS9GobPO0QlEinhTVaXWb1_uGfhzkXuULcrlafj1b20P_BVsJx29t46bwdHjGwYf5iaPho8q4Z5SvMq4V10CXpGMyw1IeaCm1SIGcaCCUCdBKL1PsJTkqq1IfEyqknzHDhPLThDPY-Eyp0FUC-KRMpbHIfDRBfNOX2YghPEHzxf80n0VO0VB3B2OV7G4HHBgPWIl1hv0GQ6UUy7jQENx5gfCECTWWpGGhRd6BmSdzrKPPMe7D5K7QFcGta5GTEQXx8H43sScY1ibzApjjVQ-Iu2lGWFkkmEBlcp3pCAC9q-w9APv1f5_5hjzyUHTjMNvjJ-Sorff6LbCmNp2RB6fLi-2XWbfqANsND2fdm_ML05Ad7Q
link.rule.ids 230,315,730,783,787,867,888,2109,2228,21402,24332,27938,27939,33758,43819,53806,53808,74638
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb5wwELXaRFV7qfpd2qS1ql5pANtgThVJdkva3VVUJVVuCIyd5bCQAhupP6T_tzPAbkJa9cIBA0aasefZfvOGkI8mdZRhPnKqGLc5S0M7hDhiu4FxmKdcLRkmOM8XfnzOv16Ii2HDrRlolZs5sZuo80rhHvmBJxiqQXmB_Hz108aqUXi6OpTQuE92UaoKvHr3cLI4_b7dZUHVS8AUfeoOg_X9wQp5bn3FpGbVhODDfsBHQanT7odQs0Rm5N-w8y578lY4mj4hjwccSaPe8E_JPV0-Iw_6ypK_npPfxz2FDlrp6U1uAK0MTekcmjB3kU6RvmZPymVHA6DR5SX6Yb8_SJFeX61bWpT0WONRAwQ5OsEUjpr-KOCX6BddVivd0KqmMXRFo7ItsgppifhSVGfVdVGvG3oyEL7KF-R8Ojk7iu2hAoOthOO3tnEzN2A85xDF_NTRMK0y7hnlq5xrxTUAptAxuWEZD3QYapEBPNEAKVMAll6u2EuyU1alfk2oCP2cGSaUn6WcwcVnSklXCUCUYRYai3zamCC56oU2EligoM2Sf9rMIodoqO3DqJPd3YAHk2HYJTrHioNSKcVyLjQs77xAeMJIjaI0TFrkA5h59I04miV4D9O7pCuCa9ciexsvSIYR3iQ3_miRV71DbD8Dy36UPmMWCUauMupn3FIWy07bWyJElPLN_7t8Tx7GZ_NZMjtZfHtLHnnIs3GY7fE9stPWa70PQKnN3g2j4Q-BOBXX
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fb9MwELagE4gXxM8RGGAhXkOTnJ04TyijLR1sVYUY2luUOPaahyYjaSfxh_D_ctekHQXESx5sJ450d77P9nd3jL21macthMSpAuEKyGI3Rj_i-pH1INC-UUABzmezcHouPl3Ii57_1Pa0yu2auFmoi1rTGfkwkEDZoIJIDW1Pi5iPJu-vvrtUQYpuWvtyGrfZQSRQqwbs4Hg8m3_ZnbhQBkzEF10YD-Bef7gkzltXPaldtjHqcxiJPQe1yeOPbmdBLMm_IeifTMrfXNPkAbvfY0qedErwkN0y1SN2p6sy-eMx-znq6HTYy-c3cQK8tjzjZ9hFcYx8QlQ2d1wtNpQAnlxekk52Z4WcqPb1esXLio8MXTugw-NjCudo-LcSf4l_NFW9NC2vGz7FqXhSrcq8JooivZQ0eX1dNuuWn_Tkr-oJO5-Mv36Yun01BldLL1y51s_9CEQh0KOFmWdwiQURWB3qQhgtDIKn2LOFhVxEJo6NzBGqGISXGYLMoNDwlA2qujLPGJdxWIAFqcM8E4CPELRWvpaILuM8tg57txVBetUl3Uhxs0IyS_8pM4cdk6B2gyln9qYBB6a9CaamoOqDSmsNhZAGt3pBJANplaEENaAc9gbFvPeNaXKaUhuFeilfRte-w462WpD21t6mN7rpsMNOIXafAVCUBg0cFu2pyt48-z1Vudjk-VYEF5V6_v8pX7O7aAjp6cns8wt2LyDKjQduII7YYNWszUvETKv8VW8MvwBTABoL
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Diagnostic+Performance+of+a+Magnetic+Field-Enhanced+Agglutination+Readout+in+Detecting+Either+Viral+Genomes+or+Host+Antibodies+in+Arbovirus+Infection&rft.jtitle=Microorganisms+%28Basel%29&rft.au=Leon%2C+Fanny&rft.au=Pinchon%2C+Elena&rft.au=Temurok%2C+Nevzat&rft.au=Morvan%2C+Fran%C3%A7ois&rft.date=2021-03-24&rft.pub=MDPI&rft.issn=2076-2607&rft.volume=9&rft.issue=4&rft_id=info:doi/10.3390%2Fmicroorganisms9040674&rft_id=info%3Apmid%2F33805173&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=oai_HAL_hal_03198157v1
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2076-2607&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2076-2607&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2076-2607&client=summon