Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test

• Published in: Micromachines (Basel) Vol. 13; no. 9; p. 1549
• Main Authors: Martinović, Rajko, Joksimović, Danijela, García-March, José Rafael, Vicente, Nardo, Gačić, Zoran
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.09.2022; MDPI
• Subjects: Aquariums; Climate change; Coefficient of variation; Endangered & extinct species; Environmental conditions; Environmental Sciences; Evaluation; Fiber lasers; Fiber optics; Heart rate; hyposalinity; Infrared detectors; Laboratories; Mollusks; Mussels; pen shell; Physiology; Pinna nobilis; Recording; Recovery time; Salinity; Seawater; Sediments; Adriatic Sea; Montenegro; Spain; Russia; hyposalinity; physiology; heart rate; mussels; pen shell; Pinna nobilis
• ISSN: 2072-666X; 2072-666X
• DOI: 10.3390/mi13091549

A non-invasive laser fiber-optic method based on infrared sensors for heart rate (Hr) recording was applied to assess the physiological condition of . During 2017, the specimens of were sampled at three sites within the Boka Kotorska Bay, Montenegro and used for ex situ experiments with short-term reduction/restoration of ambient salinity to evaluate their physiological adaptive capacity based on heart rate recovery time (T ). Mean T for specimens from Sv. Nedjelja (reference site), Dobrota and Sv. Stasije were 72 ± 3, 91 ± 7 and 117 ± 15 min, while the coefficients of variation (CV) were 0.12, 0.13 and 0.17, respectively. Resting heart rate (Hr ) and T showed statistically significant differences between the groups of mussels from Dobrota and Sv. Stasije in comparison to the reference site. Statistically significant correlations were observed between T and shell length/width, which was not the case in comparison between Hr and shell length/width. The lower adaptive capacity within the specimens from Dobrota and Sv. Stasije in comparison to the reference site could occur due to stress induced by deterioration of environmental conditions, which could have led to impairment of the physiological state of the mussels evaluated by Hr. All the specimens of survived the experimental treatments; afterwards, they were successfully transplanted at the Dobrota site. The experimental unit with sensor technology applied in this study can provide Hr recording in real time and could have an application in monitoring the physiological/health state of individuals maintained in aquaria.