Intracellular Inositol(1,4,5)-Trisphosphate Receptor Levels Are Preserved in Alzheimer’s Disease Platelets

• Published in: Neurobiology of aging Vol. 18; no. 5; pp. 559 - 561
• Main Authors: Garlind, A, Wiehager, B, Winblad, B, Fowler, C.J
• Format: Journal Article
• Language: English
• Published: London Elsevier Inc 01.09.1997; Elsevier Science
• Subjects: Aged; Aged, 80 and over; Alzheimer Disease - blood; Alzheimer’s disease; Biological and medical sciences; Blood Platelets - metabolism; Blood Platelets - ultrastructure; Calcium - blood; Calcium Channels - metabolism; Cell Membrane - metabolism; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Female; Humans; Inositol 1,4,5-Trisphosphate Receptors; Inositol(1,4,5)trisphosphate receptor; Male; Medical sciences; Medicin och hälsovetenskap; Neurology; Platelets; Receptors, Cytoplasmic and Nuclear - metabolism; Alzheimer’s disease; Platelets; Inositol(1,4,5)trisphosphate receptor; Human; Signal transduction; Nervous system diseases; Platelet; Alzheimer disease; Central nervous system disease; Degenerative disease; Inositol phosphate; Cerebral disorder; Biological receptor
• ISSN: 0197-4580; 1558-1497
• DOI: 10.1016/S0197-4580(97)00039-0

An increasing number of signal transduction disturbances have been reported in Alzheimer’s disease. These changes are not restricted to histopathologically changed brain areas but are seen also in peripheral tissues. One of the most severe disturbances is a loss of calcium-mobilizing intracellular inositol(1,4,5)-trisphosphate receptors in Alzheimer cerebellar and cortical tissues. In the present study, the binding of [ 3H]inositol(1,4,5)trisphosphate ([ 3H]Ins(1,4,5)P 3) to the calcium-mobilizing inositol(1,4,5)trisphosphate receptors in platelet membranes from eight Alzheimer’s disease patients and eight control subjects were investigated to determine its possible role as a biological marker in Alzheimer’s disease. It was found that there were no significant difference in [ 3H]Ins(1,4,5)P 3 binding with respect to the number of sites measured at different protein concentrations or to the sensitivity of the binding to inhibition by nonradioactive Ins(1,4,5)P 3 between Alzheimer disease platelets and controls. It is concluded that inositol(1,4,5)trisphosphate receptor levels are preserved in platelets from patients with Alzheimer’s disease.