Chryseobacterium miricola sp. nov., A Novel Species Isolated from Condensation Water of Space Station Mir

• Published in: Systematic and applied microbiology Vol. 26; no. 4; pp. 523 - 528
• Main Authors: Li, Ying, Kawamura, Yoshiaki, Fujiwara, Nagatoshi, Naka, Takashi, Liu, Hongsheng, Huang, Xinxiang, Kobayashi, Kazuo, Ezaki, Takayuki
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.11.2003; Elsevier; Elsevier Science Ltd
• Subjects: 16S rDNA; Action of physical and chemical agents; Bacteriology; Base Composition; Biological and medical sciences; Chryseobacteirum miricola sp. nov; Chryseobacterium - classification; Chryseobacterium - cytology; Chryseobacterium - isolation & purification; Chryseobacterium - physiology; Chryseobacterium meningosepticum; Chryseobacterium miricola; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - isolation & purification; Fatty Acids - analysis; Fatty Acids - chemistry; Flavobacteria; Flavobacteriaceae; Freshwater; Fundamental and applied biological sciences. Psychology; Microbiology; Miscellaneous; Molecular Sequence Data; Movement; Nucleic Acid Hybridization; Phenotype; Phylogeny; Pigments, Biological - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; space station; Spacecraft; Spores, Bacterial; Virology; Vitamin K 2 - analysis; Vitamin K 2 - chemistry; Water Microbiology; 16S rDNA; Chryseobacteirum miricola sp. nov; Flavobacteria; space station; Chryseobacterium meningosepticum; Flavobacteriaceae; Nucleotide sequence; Quinone; Ribosomal DNA; Lipids; Fatty acids; IS element; Strain; 16S-RNA; Bacteria; Chryseobacteirium miricola sp. nov; Gram negative bacteria
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/072320203770865828

Classification of strain W3-B1, which was isolated from condensation water in the Russian space laboratory Mir, was investigated by a polyphasic taxonomic approach. Cells of strain W3-B1 were nonmotile, asporogenous, gram-negative slender rods with rounded ends. 16S rRNA gene sequence analysis indicated that organism should be placed in the genus Chryseobacterium. This organism contains menaquinone MK-6 as the predominent isoprenoid quinone and 3-OH iso17:0 (40%), iso15:0 (33%) as the major fatty acids. Phylogenetically, the nearest relative of strain W3-B1 is Chryseobacterium meningosepticum with sequence similarity of 98.4%, but DNA-DNA hybridization resulted in similarity values of only 52.3%. The G+C mol% is 34.6 mol%. Based upon results obtained by morphological, biochemical, chemotaxonomic, and molecular methods, strain W3-B1 was clearly distinguishable from other Chryseobacterium species. For these reasons, a novel species of family Flavobacteriaceae is proposed; strain W3-B1 T (= GTC 862 T = JCM 11413 T = DSM 14571 T) is the type strain.