Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4

• Published in: Current research in microbial sciences Vol. 1; pp. 44 - 52
• Main Authors: Havryliuk, Olesia, Hovorukha, Vira, Patrauchan, Marianna, Youssef, Noha H., Tashyrev, Oleksandr
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2020; Elsevier
• Subjects: Antarctic, and Ukrainian soils; Arctic; Environmental biotechnologies; Short Communication; Soil isolates; Сopper-resistant microorganisms; Soil isolates; Antarctic, and Ukrainian soils; Arctic; Environmental biotechnologies; Сopper-resistant microorganisms
• ISSN: 2666-5174; 2666-5174
• DOI: 10.1016/j.crmicr.2020.06.002

•Four bacterial strains with record resistance to Cu(II) have been isolated.•Genomic sequences are available for genome mining and studying the genetic determinants of bacterial resistance to Cu(II).•Genomic sequences provide the foundation necessary for transcriptional and functional studies of genes encoding for Cu(II) resistance mechanisms in Pseudomonas spp.•The data will be of interest for a broad community of biotechnologists and microbiologists and will aid in developing novel technologies for copper detoxification in contaminated soils and industrial wastewaters. Environmental copper pollution causes major destruction to ecological systems, which require the development of environmentally friendly biotechnological, in particular, microbial methods for copper removal. These methods rely on the availability of microorganisms resistant to high levels of copper. Here we isolated four bacterial strains with record resistance to up to 1.0 M Cu(II). The strains were isolated from ecologically diverse soil samples, and their genomes were sequenced. A 16S rRNA sequence-based phylogenetic analysis identified that all four isolates belong to the genus Pseudomonas. Particularly, strains UKR1 and UKR2 isolated from Kyiv region in Ukraine were identified as P. lactis and P. panacis, respectively, and strains UKR3 and UKR4 isolated from Svalbard Island in the Arctic Ocean and Galindez Island in Antarctica, respectively, were identified as P. veronii. Initial in-silico screening for genes encoding copper resistance mechanisms showed that all four strains encode copper resistance proteins CopA, CopB, CopD, CopA3, CopZ, as well as two-component regulatory system CusRS, all known to be associated with metal resistance in Pseudomonas genus. Further detailed studies will aim to characterize the full genomic potential of the isolates to enable their application for copper bioremediation in contaminated soils and industrial wastewaters. [Display omitted]