Mammalian embryo comparison identifies novel pluripotency genes associated with the naïve or primed state
During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, 'naïve' or 'primed', depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene express...
Saved in:
Published in | Biology open Vol. 7; no. 8 |
---|---|
Main Authors | , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
The Company of Biologists Ltd
2018
Royal Society The Company of Biologists |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, 'naïve' or 'primed', depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states
and
Amongst these are the pluripotency-linked genes
and
The novel genes discovered include naïve- (
and
) and primed-associated (
and
) genes as well as naïve to primed transition genes (
and
). Both
and
play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC6124576 Joint second authors Joint first authors |
ISSN: | 2046-6390 2046-6390 |
DOI: | 10.1242/bio.033282 |