Role of heavy metal resistant Ochrobactrum sp. and Bacillus spp. strains in bioremediation of a rice cultivar and their PGPR like activities

• Published in: The journal of microbiology Vol. 51; no. 1; pp. 11 - 17
• Main Authors: Pandey, S., Burdwan University, West Bengal, India, Ghosh, P.K., Burdwan University, West Bengal, India, Shosh, S., Burdwan University, West Bengal, India, De, T.K., Calcutta University, Calcutta, India, Maiti, T.K., Burdwan University, West Bengal, India
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.02.2013; The Microbiological Society of Korea; Springer Nature B.V; 한국미생물학회
• Subjects: 1-aminocyclopropane-1-carboxylate deaminase; 1-aminocyclopropane-1-carboxylic acid; Amylases - metabolism; ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; Arsenic; Bacillus; Bacillus (bacteria); Bacillus - drug effects; Bacillus - metabolism; Bacteria; BIODECONTAMINACION; Biodegradation, Environmental; Biomass; Biomedical and Life Sciences; BIOREMEDIATION; Cadmium; catalase; Cultivars; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Drug Resistance, Bacterial; enzyme activity; germination; Germination - drug effects; growth promotion; Heavy metals; host plants; Hydroponics; inoculum; lead; Life Sciences; malondialdehyde; metal tolerance; Metals, Heavy - metabolism; Metals, Heavy - toxicity; Microbial Ecology and Environmental Microbiology; Microbiology; Molecular Sequence Data; Ochrobactrum; Ochrobactrum - drug effects; Ochrobactrum - metabolism; Oryza - drug effects; Oryza - growth & development; Oryza - microbiology; Oryza - physiology; Oryza sativa; Peptide Hydrolases - metabolism; Plant growth; plant growth-promoting rhizobacteria; Plant Roots - drug effects; Plant Roots - growth & development; proteinases; rice; root growth; roots; Seeds; Sequence Analysis, DNA; shoots; SIDEROFOROS; SIDEROPHORE; SIDEROPHORES; Soil contamination; starch; stress ethylene,metal tolerance,free-radicals,ACC deaminase; superoxide dismutase; Toxicity; 생물학; West Bengal India; India; Kolkata India; antioxidant; bioremediation; free-radicals; ACC deaminase; stress ethylene; metal tolerance; siderophores
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-013-2330-7

The present study demonstrates the metal toxicity ameliorating and growth promoting abilities of three different bacterial isolates when applied to rice as host plant. The three bacterial strains included a cadmium resistant Ochrobactrum sp., a lead resistant Bacillus sp. and an arsenic resistant Bacillus sp. designated as CdSP9, PbSP6, and AsSP9, respectively. When these isolates were used as inocula applied to metal-treated rice plants of variety Satabdi, the germination percentage, relative root elongation (RRE), amylase and protease activities were increased. The toxic effect of metal was reduced in presence of these bacteria. The overall biomass and root/shoot ratio were also enhanced by bacterial inoculation. Hydroponic studies showed that the superoxide dismutase (SOD) activity and malondialdehyde (MDA) level, which had been increased in the presence of metal stress in rice roots, were lowered by the bacterial inoculation. In addition, all three strains were 1-aminocyclopropane-1-carboxylate (ACC) deaminase and catalase positive, whereas siderophore producing ability was lacking in PbSP6. However, both PbSP6 and AsSP9 were protease positive and could hydrolyse starch. The data indicate that these bacteria have promise for bioremediation as well as for plant growth promotion.