Bioremediation of malachite green dye by two bacterial strains isolated from textile effluents

• Published in: Current research in microbial sciences Vol. 1; pp. 37 - 43
• Main Authors: Roy, Dipankar Chandra, Biswas, Sudhangshu Kumar, Sheam, Md. Moinuddin, Hasan, Md Rockybul, Saha, Ananda Kumar, Roy, Apurba Kumar, Haque, Md. Enamul, Rahman, Md. Mizanur, Tang, Swee-Seong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2020; Elsevier
• Subjects: 16S rRNA gene sequencing; Bioremediation; Enterobacter sp; Malachite Green dye; RAPD; Research Paper; Textile effluents; Enterobacter sp; Textile effluents; 16S rRNA gene sequencing; Bioremediation; Malachite Green dye; RAPD
• ISSN: 2666-5174; 2666-5174
• DOI: 10.1016/j.crmicr.2020.06.001

•About 280,000 tons of fabric dyes are emitted to the industrial effluents annually.•These synthetic dyes are hazardous for aquatic environment and public health.•Biodegradation of such non-degradable colorants can be an eco-friendly approach.•Two MG dye degrading bacteria had been identified by RAPD and 16S rRNA sequencing.•These bacteria of Enterobacter spp. can degrade 100% Malachite Green dye (15 mg/L). Globally, water pollution from the textile industries is an alarming issue. Malachite Green dye of the triphenylmethane group is an extensively used dye in the fabric industries that is emitted through textile wastewater. This study aimed to isolate and characterize potential Malachite Green (MG) dye degrading bacteria from textile effluents. Different growth and culture parameters such as temperature, pH and dye concentration were optimized to perform the dye-degradation assay using different concentrations of MG dye in the mineral salt medium. A photo-electric-colorimeter was used to measure the decolorizing activity of bacteria at different time intervals after aerobic incubation. Two potential bacterial strains of Enterobacter spp. CV-S1 (accession no: MH450229) and Enterobacter spp. CM-S1 (accession no: MH447289) were isolated from textile effluents exhibiting potential MG dye decoloring efficiency. Further, the RAPD analysis and 16S rRNA sequencing confirmed the genetic differences of the isolated strains. Enterobacter sp CV-S1 and Enterobacter sp CM-S1 can completely decolor MG dye up to 15 mg/L under shaking condition without any requirement of sole carbon source. Thus, these two bacteria have the potency to be utilized in the textile wastewater treatment plant. [Display omitted]