Molecular biological characterization of equine herpesvirus type 1 (EHV-1) isolates from ruminant hosts

• Published in: Virus research Vol. 11; no. 2; p. 127
• Main Authors: Chowdhury, S I, Ludwig, H, Buhk, H J
• Format: Journal Article
• Language: English
• Published: Netherlands 01.09.1988
• Subjects: Animals; antelopes; Antelopes - microbiology; antilope; Artiodactyla - microbiology; biologia molecular; biologie moleculaire; bovin; cattle; Cattle - microbiology; DNA Probes; DNA, Viral - genetics; DNA, Viral - isolation & purification; equine herpesvirus; ganado bovino; Herpesviridae - isolation & purification; Herpesvirus 1, Equid - genetics; Herpesvirus 1, Equid - isolation & purification; herpesvirus equin; herpesvirus equino; molecular biology; Neutralization Tests; Nucleic Acid Hybridization; Restriction Mapping
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/0168-1702(88)90038-x

Isolates of equine herpesvirus type 1 (EHV-1) originating from affected antelope and cattle were compared with reference EHV-1 and EHV-4 isolates and were characterized. Based on cross-neutralization, DNA restriction profiles and blot-hybridization data these isolates could be characterized as EHV-1. One isolate (from an antelope) with a different restriction profile showed significant DNA homology with EHV-1, partial homology with EHV-4, and little or no homology with EHV-2 and HSV-1 DNAs. Blot hybridization revealed differences in DNA restriction fragments located at the termini of two isolates and size heterogeneity in the unique long/internal repeat junction fragment (UL/IR) of one isolate.