Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi Epimastigotes

• Published in: The Journal of biological chemistry Vol. 266; no. 35; pp. 23670 - 23675
• Main Authors: DE LEDERKREMER, R. M, LIMA, C, RAMIREZ, M. I, FERGUSON, M. A. J, HOMANS, S. W, THOMAS-OATES, J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 15.12.1991
• Subjects: Acylation; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Carbohydrate Sequence; Carbohydrates; Chromatography, High Pressure Liquid; Fundamental and applied biological sciences. Psychology; Heterosides; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Oligosaccharides - chemistry; Oligosaccharides - isolation & purification; Other biological molecules; Peptidoglycan - chemistry; Peptidoglycan - isolation & purification; Phospholipids - chemistry; Phospholipids - isolation & purification; Spectrometry, Mass, Fast Atom Bombardment; Trypanosoma cruzi; Trypanosoma cruzi - chemistry; Protozoa; Rhizoflagellata; HPLC chromatography; Primary structure; NMR spectrometry; Inositol phosphate; Mass spectrometry; Glycan; Trypanosoma cruzi
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/s0021-9258(18)54336-2

The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J. O., Gorin, P. A. J., Mazurek, M., Xavier, M. T., Fournet, B., Wieruszesk, J. M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.