NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of Vibrio diabolicus CNCM I-1629

• Published in: Marine drugs Vol. 20; no. 12; p. 731
• Main Authors: Drouillard, Sophie, Poulet, Laurent, Boisset, Claire, Delbarre-Ladrat, Christine, Helbert, William
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.11.2022; MDPI
• Subjects: Bacteria; Bacterial Proteins - chemistry; Bacteroides - enzymology; Carbon; Chromatography; Communication; diabolican; Enzymes; exopolysaccharide; Exopolysaccharides; Glycosaminoglycans; hyaluronan lyase; Hyaluronic acid; Life Sciences; Molecular structure; Molecular weight; NMR; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular; Oligosaccharides; Oligosaccharides - chemistry; Pathogens; Polysaccharide-Lyases - chemistry; Polysaccharides; Polysaccharides, Bacterial - chemistry; Size exclusion chromatography; Substrates; Vibrio - chemistry; Vibrio diabolicus; exopolysaccharide; diabolican; NMR; hyaluronan lyase
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20120731

Diabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium (CNCM I-1629). This polysaccharide was enzymatically degraded by the WH2 hyaluronan lyase. The end products were purified by size-exclusion chromatography and their structures were analyzed in depth by nuclear magnetic resonance (NMR). The oligosaccharide structures confirmed the possible site of cleavage of the enzyme showing plasticity in the substrate recognitions. The production of glycosaminoglycan-mimetic oligosaccharides of defined molecular weight and structure opens new perspectives in the valorization of the marine polysaccharide diabolican.