Comparative drug susceptibility study of five clonal strains of Trichomonas vaginalis in vitro

• Published in: Asian Pacific journal of tropical medicine Vol. 4; no. 1; pp. 50 - 53
• Main Authors: Chaudhari, Hemantkumar Somabhai, Singh, Prati Pal
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 2011; Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research NIPER, Sector-67, Phase-X, S.A.S.Nagar - 160062, Punjab, India
• Subjects: Antiprotozoal Agents - pharmacology; Azoles - pharmacology; Clonal; Clonal strains; Drug; Drug susceptibility; Humans; Metronidazole; Parasitic Sensitivity Tests; strains; susceptibility; Thiazoles - pharmacology; Trichomonas; Trichomonas vaginalis; Trichomonas vaginalis - drug effects; vaginalis; Trichomonas vaginalis; Clonal strains; Drug susceptibility; Metronidazole
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/S1995-7645(11)60031-X

<正>Objective:To produce comparative data on a group of Trichomonas vaginalis clonal strains with varied drug responses using identical methods and materials.Methods:Five clonal strains of Trichomonas vaginalis were isolated from reference strain using agar plate technique.The variability of growth kinetic and susceptibility of clonal strain to metronidazole,tinidazole, satranidazole and nitazoxanide were observed in 96 well microlilre plate.Results:Among these clonal strains there was a good correlation between rates of growth with the relative susceptibility of the strains to drugs in vitro.Regarding metronidazole,tinidazole and satranidazole susceptibility,different degrees of susceptibility were determined.However,no difference in nitazoxanide susceptibility was found between the clonal strain tested and a reference strain. Conclusions:This is the first description of biological variability in clonal stock of Trichomonas vaginalis.Different degrees of drug susceptibility were determined among clonal strains tested. Further studies will be necessary to ascertain the importance of this variability in clinical infection.