In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen

• Published in: Theriogenology Vol. 73; no. 4; pp. 480 - 487
• Main Authors: Forouzanfar, M., Sharafi, M., Hosseini, S.M., Ostadhosseini, S., Hajian, M., Hosseini, L., Abedi, P., Nili, N., Rahmani, H.R., Nasr-Esfahani, M.H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2010; [Oxford]: Butterworth-Heinemann; [New York]: Elsevier Science
• Subjects: Acrosome Reaction; Acrosome Reaction - physiology; Animals; capacitation; Capacitation status; cell cleavage; chemistry; Cryopreservation; Cryopreservation - methods; Cryopreservation - veterinary; Cryoprotective Agents; Egg Yolk; Extenders; Fertilization in Vitro; Fertilization in Vitro - veterinary; Glycine max - chemistry; in vitro studies; IVF; Lecithins; Male; methods; phosphatidylcholines; physiology; Ram semen; rams; semen; semen extenders; Semen Preservation; Semen Preservation - methods; Semen Preservation - veterinary; Sheep; soybean lecithin; soybean products; Soybeans; sperm motility; Sperm Motility - physiology; sperm viability; Spermatozoa; veterinary; viability; IVF; Ram semen; Capacitation status; Extenders; Cryopreservation
• ISSN: 0093-691X; 1879-3231; 1879-3231
• DOI: 10.1016/j.theriogenology.2009.10.005

Substitution of egg yolk with soybean lecithin may reduce hygienic risks in extenders. Though a few studies have been performed on the effect of soybean lecithin in bull, to date evaluation of ram semen in vitro fertility after cryopreservation with use of soybean lecithin has not been studied. This study assessed the effect of 1% or 2% (wt/vol) soybean lecithin (L1 or L2) or 15% or 20% (vol/vol) egg yolk (E15 or E20) supplemented with 5% or 7% glycerol (G5 or G7) in a Tris-based medium for cryopreservation of ram (Oviss arries) semen. Although no significant difference was observed in pattern of capacitation, the best results in terms of sperm motility, viability postthaw, and cleavage rates were observed with L1G7 (51.9 ± 4.8%, 48.1 ± 3.5%, and 79.6 ± 3.9%, respectively) and E20G7 (51.8 ± 2.9%, 46.7 ± 4.0%, and 72.9 ± 6.4%, respectively). Our results also showed that 1% lecithin and 20% egg yolk was superior to 2% lecithin and 15% egg yolk. In terms of cleavage rate, 7% glycerol was superior to 5% glycerol. No significant difference was obtained between groups in terms of blastocysts rate per cleaved embryo. Therefore, we concluded that the optimal concentration of lecithin and egg yolk is 1% and 20%, respectively, along with 7% glycerol. In addition, our results suggest that lecithin can be used as a substitute for egg yolk.