The First Report of Vibrio parahaemolyticus Strain O10:K60 in Japan, a New Combination of O and K Serotypes Isolated from a Patient with Gastroenteritis

• Published in: Japanese Journal of Infectious Diseases Vol. 69; no. 1; pp. 28 - 32
• Main Authors: Ueno, Hiroyuki, Tomari, Kentaro, Kikuchi, Koji, Kobori, Sumie, Miyazaki, Motonobu
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 01.01.2016
• Subjects: Adolescent; Anti-Bacterial Agents - pharmacology; Antigens, Bacterial - analysis; Antigens, Surface - analysis; Female; Gastroenteritis - diagnosis; Gastroenteritis - microbiology; Humans; Japan; Microbial Sensitivity Tests; O Antigens - analysis; Polymerase Chain Reaction; Serogroup; serotyping; the pandemic clone; Vibrio Infections - diagnosis; Vibrio Infections - microbiology; Vibrio parahaemolyticus; Vibrio parahaemolyticus - classification; Vibrio parahaemolyticus - drug effects; Vibrio parahaemolyticus - genetics; Vibrio parahaemolyticus - isolation & purification; Virulence Factors - genetics
• ISSN: 1344-6304; 1884-2836
• DOI: 10.7883/yoken.JJID.2014.538

Vibrio parahaemolyticus is an important pathogen that causes gastroenteritis in humans, generally associated with the consumption of contaminated seafood, particularly raw shellfish. There are many serotypes in V. parahaemolyticus resulting from a combination of O and K antigens. Among them, O3:K6 and their variants, which represent the pandemic clone, are the most widespread strains worldwide. In this study, we examined V. parahaemolyticus isolated from a gastroenteritis patient's stool at a hospital in Saitama City, Japan in 2013. Serotyping of the O and K antigens identified the strain as O10:K60. To our knowledge, this is the first reported case of a V. parahaemolyticus strain with this antigen combination in Japan. Subsequently, we used PCR to assay for pathogenicity-associated genes, and found that it was positive for tdh, T3SS1, and T3SS2α genes. Antibiotic susceptibility tests showed that the strain was susceptible to all selected antibiotics except ampicillin. Moreover, we detected specific marker genes for the pandemic clone with two kinds of PCR assay. Our results suggest that the isolate O10:K60 is a newly emerging serotype that belongs to the pandemic clone.