Molecular characterization of diapause hormone and pheromone biosynthesis activating neuropeptide from the black-back prominent moth, Clostera anastomosis (L.) (Lepidoptera, Notodontidae)

• Published in: Insect biochemistry and molecular biology Vol. 37; no. 12; pp. 1262 - 1271
• Main Authors: Jing, Tian-Zhong, Wang, Zhi-Ying, Qi, Feng-Hui, Liu, Kuan-Yu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2007
• Subjects: Agrotis ipsilon; Amino Acid Sequence; amino acid sequences; Animals; Base Sequence; Biological Evolution; Bombycoidea; chemistry; Clostera anastomosis; complementary DNA; Developmental expression; developmental stages; diapause hormone; Diapause hormone (DH); exons; Female; gene expression; Gene Expression Regulation, Developmental; Genes, Insect; genetics; Helicoverpa armigera; Helicoverpa assulta; Helicoverpa zea; introns; Lepidoptera; Male; messenger RNA; metabolism; molecular cloning; Molecular Sequence Data; Molecular Structure; Moths; Moths - chemistry; Moths - genetics; Moths - metabolism; Multigene Family; Neuropeptides; Neuropeptides - chemistry; Neuropeptides - genetics; Neuropeptides - metabolism; Noctuidae; Noctuoidea; Notodontidae; nucleotide sequences; pheromone biosynthesis activating neuropeptide; Pheromone biosynthesis activating neuropeptide (PBAN); phylogeny; Sequence Analysis, DNA; sequence homology; Tortricoidea; Yponomeutoidea; Developmental expression; Pheromone biosynthesis activating neuropeptide (PBAN); Diapause hormone (DH); Clostera anastomosis
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2007.07.012

Using a strategy of rapid amplification of cDNA ends, the cDNA of diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN) was cloned from the head of Clostera anastomosis (L.). The Cloan-DH-PBAN cDNA contains an open reading frame encoding a 196-amino acid preprohormone, from which five putative FXPRL peptides, DH, PBAN, α-SGNP(SGNP, suboesophageal ganglion neuropeptide), β-SGNP and γ-SGNP, are released. Comparing the deduced amino acid sequences from cDNAs of these five FXPRL peptides to those known from other insects, Cloan-DH shows highest similarity of 93.1% to that from Agrotis ipsilon, Cloan-PBAN 93.9% to those from Helicoverpa armigera, Helicoverpa zea and Helicoverpa assulta, which show the highest similarity to species of Noctuidae. Phylogenetic analysis revealed that the Cloan-DH-PBAN gene is relatively closely related to those from Noctuoidea, but distant from those from Tortricoidea, Yponomeutoidea and Bombycoidea species. The DNA sequence encoding Cloan-DH-PBAN was cloned by PCR, which is 3698 bp in size and comprises six exons interspersed by five introns. Developmental expression of the DH-PBAN transcripts in the head was also showed by a semi-quantitative RT-PCR method, which was relatively low in larvae and remained low in pupae of both sexes, increased sharply in adults of both sexes.