Marine Transcriptomics Analysis for the Identification of New Antimicrobial Peptides

• Published in: Marine drugs Vol. 19; no. 9; p. 490
• Main Authors: Houyvet, Baptiste, Bouchon-Navaro, Yolande, Bouchon, Claude, Corre, Erwan, Zatylny-Gaudin, Céline
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.08.2021; MDPI
• Subjects: Amino acids; Animals; Anti-Bacterial Agents - pharmacology; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial peptides; Antimicrobial Peptides - genetics; Antimicrobial Peptides - pharmacology; Aquaculture; Bacteria; Bacteria - drug effects; Bacteria - growth & development; Cathelicidins; Defensins; Erythrocytes - drug effects; Fish; Fish Proteins - genetics; Fish Proteins - pharmacology; Gene Expression Profiling; Genes; Gram-negative bacteria; Gram-positive bacteria; Hemolysis - drug effects; Histones; Humans; Immune system; Immunity; Immunosuppressive agents; Life Sciences; lionfish; Liver; Marine fishes; Microorganisms; Molecular weight; Pathogens; Peptides; Perciformes - genetics; Pterois; Transcription; Transcriptome; Transcriptomes; transcriptomic; Trout; venomous fish; Zebrafish; Caribbean Sea; lionfish; transcriptomic; antimicrobial peptides; venomous fish
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md19090490

Antimicrobial peptides (AMPs) participate in the immune system to avoid infection, are present in all living organisms and can be used as drugs. Fish express numerous AMP families including defensins, cathelicidins, liver-expressed antimicrobial peptides (LEAPs), histone-derived peptides, and piscidins (a fish-specific AMP family). The present study demonstrates for the first time the occurrence of several AMPs in lionfish ( ). Using the lionfish transcriptome, we identified four transcript sequences encoding cysteine-rich AMPs and two new transcripts encoding piscidin-like peptides. These AMPs are described for the first time in a species of the Scorpaenidae family. A functional approach on new pteroicidins was carried out to determine antimicrobial sequences and potential uses, with a view to using some of these AMPs for human health or in aquaculture.