Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK

• Published in: Journal of industrial microbiology & biotechnology Vol. 40; no. 3-4; pp. 393 - 397
• Main Authors: Hou, Lihua, Li, Xiaoyang, Wang, Cong, Cao, Xiaohong, Wang, Haiyong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2013; Springer; Springer Nature B.V
• Subjects: Biochemistry; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cloning; Deoxyribonucleases, Type II Site-Specific - genetics; Ethanol; Fermentation; Food science; Fundamental and applied biological sciences. Psychology; genes; Genes, Fungal; Genetic Engineering; industrial applications; Inorganic Chemistry; Life Sciences; Microbiology; Mutagenesis; Mutation; Plasmids; Plasmids - genetics; Ploidies; ploidy; Polyploidy; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Short Communication; Studies; Yeast; China; Polyploidy; Plac33-GHK; Yeast; Ascomycota; Fungi; Plasmid; Ploidy; Saccharomyces cerevisiae

An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial a...