Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK
An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial a...
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Published in | Journal of industrial microbiology & biotechnology Vol. 40; no. 3-4; pp. 393 - 397 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer-Verlag
01.04.2013
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes. |
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Bibliography: | http://dx.doi.org/10.1007/s10295-013-1241-2 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1367-5435 1476-5535 |
DOI: | 10.1007/s10295-013-1241-2 |