Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK

An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial a...

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Bibliographic Details
Published inJournal of industrial microbiology & biotechnology Vol. 40; no. 3-4; pp. 393 - 397
Main Authors Hou, Lihua, Li, Xiaoyang, Wang, Cong, Cao, Xiaohong, Wang, Haiyong
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.04.2013
Springer
Springer Nature B.V
Subjects
Biochemistry
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cloning
Deoxyribonucleases, Type II Site-Specific - genetics
Ethanol
Fermentation
Food science
Fundamental and applied biological sciences. Psychology
genes
Genes, Fungal
Genetic Engineering
industrial applications
Inorganic Chemistry
Life Sciences
Microbiology
Mutagenesis
Mutation
Plasmids
Plasmids - genetics
Ploidies
ploidy
Polyploidy
Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - genetics
Short Communication
Studies
Yeast
China
Polyploidy
Plac33-GHK
Yeast
Ascomycota
Fungi
Plasmid
Ploidy
Saccharomyces cerevisiae
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Summary:An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes.
Bibliography:http://dx.doi.org/10.1007/s10295-013-1241-2
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ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-013-1241-2