Influence of activated charcoal, porcine gastric mucin and β-cyclodextrin on the morphology and growth of intestinal and gastric Helicobacter spp

• Published in: Microbiology (Society for General Microbiology) Vol. 148; no. Pt 3; pp. 677 - 684
• Main Authors: TANEERA, Jalal, MORAN, Anthony P, HYNES, Sean O, NILSSON, Hans-Olof, WALEED ABU AL-SOUD, WADSTRÖM, Torkel
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.03.2002
• Subjects: Animal; Animals; Bacteriological Techniques; Bacteriology; Basic Medicine; beta -Cyclodextrin; Biological and medical sciences; Charcoal; Colony Count; Colony Count, Microbial; Culture Media; Cyclodextrins; Electron; Fundamental and applied biological sciences. Psychology; Gastric Mucin; Gastric Mucins; Growth, nutrition, cell differenciation; Helicobacter; Helicobacter pylori - growth & development; Helicobacter pylori - isolation & purification; Helicobacter pylori - ultrastructure; Helicobacter pylori/growth & development/isolation & purification/ultrastructure; Human; Humans; Intestines - microbiology; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Microbial; Microbiology; Microbiology in the medical area; Microscopy; Microscopy, Electron; Mikrobiologi inom det medicinska området; mucins; Non-U.S. Gov't; Stomach - microbiology; Support; Swine; Culture medium; Cell proliferation; Mucin; Spirillales; Helicobacter; Process improvement; Pathogenic; Spirillaceae; Phenotype variation; Bacteria; Charcoal
• ISSN: 1350-0872; 1465-2080; 1465-2080
• DOI: 10.1099/00221287-148-3-677

Bile-tolerant Helicobacter spp. are emerging human and animal pathogens. However, due to their fastidious nature, which requires nutrient-rich complex media to grow, infection with these bacteria may be underestimated. The accumulation of toxic metabolites in cultures may be one of the main obstacles for successful culture of these organisms. The present study examined various potential growth-enhancing substances for Helicobacter spp. and, furthermore, how they may affect spiral to coccoid conversion. Five Helicobacter spp. were cultured on agar and in broth media supplemented with activated charcoal, beta-cyclodextrin, or porcine gastric mucin. Growth was determined by estimating the numbers of colony-forming units and colony diameter, as well as bacterial cell mass. Coccoid transformation was estimated every 24 h by both Gram and acridine-orange staining. Activated charcoal was superior in supporting growth and increased cell mass on agar and in broth media. beta-Cyclodextrin delayed spiral to coccoid conversion by Helicobacter pylori and Helicobacter canis, whereas activated charcoal delayed the conversion to coccoid forms of Helicobacter hepaticus and Helicobacter bilis. The progression to coccoid forms by Helicobacter pullorum on agar media was not influenced by any growth supplement. The spiral to coccoid conversion was more rapid in broth media than on agar media. The growth enhancement observed is probably related to the capacity of activated charcoal to remove toxic compounds in culture media.