MTOR Suppresses Cigarette Smoke-Induced Airway Inflammation and MMP12 Expression in Macrophage in Chronic Obstructive Pulmonary Disease

• Published in: International journal of chronic obstructive pulmonary disease Vol. 19; pp. 269 - 279
• Main Authors: Dong, Lingling, Wang, Yong, Chen, Haipin, Li, Zhouyang, Xu, Xuchen, Zhou, Jiesen, Shen, Huahao, Chen, Zhihua
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2024; Dove; Dove Medical Press
• Subjects: Animals; cigarette smoke; Cigarette Smoking - adverse effects; Development and progression; Humans; Inflammation; Inflammation - metabolism; Lung; Lung diseases, Obstructive; Macrophages; Macrophages - metabolism; matrix metalloproteinase 12; Matrix Metalloproteinase 12 - genetics; Matrix Metalloproteinase 12 - metabolism; Matrix Metalloproteinase 12 - pharmacology; mechanistic target of rapamycin; Mice; Mice, Inbred C57BL; mmp12; mtor; NF-kappa B - metabolism; Nicotiana; Original Research; Pneumonia - etiology; Pulmonary Disease, Chronic Obstructive - metabolism; pulmonary emphysema; Pulmonary Emphysema - complications; Respiratory agents; Smoking; TOR Serine-Threonine Kinases - adverse effects; TOR Serine-Threonine Kinases - metabolism; China; CS; MMP12; matrix metalloproteinase 12; pulmonary emphysema; mechanistic target of rapamycin; cigarette smoke; MTOR
• ISSN: 1178-2005; 1176-9106; 1178-2005
• DOI: 10.2147/COPD.S426333

Macrophage-derived matrix metalloproteinase 12 (MMP12) can cause destruction of lung tissue structure and plays a significant role in the development and progression of chronic obstructive pulmonary disease (COPD). MTOR is a serine/threonine kinase that plays a crucial role in cell growth and metabolism. The activity of MTOR in the lung tissues of COPD patients also shows significant changes. However, it is unclear whether MTOR can regulate the development and progression of COPD by controlling MMP12. This study primarily investigates whether MTOR in macrophages can affect the expression of MMP12 and participate in the progression of COPD. We tested the changes in MTOR activity in macrophages exposed to cigarette smoke (CS) both in vivo and in vitro. Additionally, we observed the effect of MTOR on the expression of MMP12 in macrophages and on lung tissue inflammation and structural damage in mice, both in vivo and in vitro, using MTOR inhibitors or gene knockout mice. Finally, we combined inhibitor treatment with gene knockout to demonstrate that MTOR primarily mediates the expression of MMP12 through the NF-κB signaling pathway. Exposure to CS can enhance MTOR activity in mouse alveolar macrophages. Inhibiting the activity of MTOR or suppressing its expression leads to increased expression of MMP12. Myeloid-specific knockout of MTOR expression can promote the occurrence of CS-induced pulmonary inflammation and emphysema in mice. Inhibiting the activity of NF-κB can eliminate the effect of MTOR on MMP12. Macrophage MTOR can reduce the expression of MMP12 by inhibiting NF-κB, thereby inhibiting the occurrence of COPD inflammation and destruction of lung tissue structure. Activating the activity of macrophage MTOR may be beneficial for the treatment of COPD.