Stimulation of dehydrogenase synthesis by cadmium in a cadmium-resistant strain of Saccharomyces cerevisiae

• Published in: Biochimica et Biophysica Acta (BBA) - General Subjects Vol. 585; no. 3; pp. 383 - 388
• Main Authors: Joho, Masanori, Matsumoto, Hideki, Tohoyama, Hiroshi, Murayama, Tetsuo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 04.07.1979; Elsevier BV
• Subjects: Cadmium; Cadmium - pharmacology; Cd 2; Cell-Free System; Cycloheximide; Cycloheximide - pharmacology; Dehydrogenase synthesis; Drug Resistance, Microbial; Oxidoreductases; Oxidoreductases - biosynthesis; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - enzymology; yeasts; Cd 2; Dehydrogenase synthesis; Saccharomyces cerevisiae
• ISSN: 0304-4165; 0006-3002; 1872-8006; 1878-2434
• DOI: 10.1016/0304-4165(79)90082-5

The activity of dehydrogenase in Saccharomyces cerevisiae was estimated by reduction of 2,3,5-triphenyltetrazolium chloride. By the adaptation of yeast to cadmium, the high activity of dehydrogenase was observed. Furthermore, the activity of dehydrogenase in Cd-resistant cells was increased by growing in medium containing CdSO 4. However, the activity of dehydrogenase was inhibited by the addition of CdSO 4 to the reaction mixture. The activity of dehydrogenase in Cd-sensitive cells was increased slightly by incubation with low concentrations of CdSO 4. High activity of dehydrogenase in Cd-resistant cells was completely negated by the addition of cycloheximide to the incubation medium. The increase of dehydrogenase activity is due partly to de novo synthesis of protein.