Biosynthesis of the cell adhesion molecule uvomorulin (E-cadherin) in Madin-Darby canine kidney epithelial cells

• Published in: The Journal of biological chemistry Vol. 266; no. 29; pp. 19672 - 19680
• Main Authors: SHORE, E. M, NELSON, W. J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 15.10.1991
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Autoradiography; Biological and medical sciences; Biological Transport; biosynthesis; Cadherins - biosynthesis; Cadherins - metabolism; cell adhesion molecules; Cell Line; Detergents; Dogs; E-cadherin; Electrophoresis, Polyacrylamide Gel; Epithelial Cells; Epithelium - metabolism; Fundamental and applied biological sciences. Psychology; Glycoproteins; Glycosylation; kidney; Kidney - cytology; Kidney - metabolism; Kinetics; MDCK cells; Octoxynol; pathways; Polyethylene Glycols; Protein Precursors - metabolism; Protein Processing, Post-Translational; Proteins; Posttranslational modification; Intracellular transport; Regulation(control); Cell line; Molecular processing; Cell adhesion molecule; Epithelial cell; Biosynthesis; Cell surface
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/s0021-9258(18)55045-6

The Ca(2+)-dependent cell adhesion molecule uvomorulin is a transmembrane glycoprotein that functions at the cell surface to regulate epithelial cell recognition and adhesion. We have investigated the temporal and spatial regulation of uvomorulin biosynthesis and cell surface expression in Madin-Darby canine kidney epithelial cells. We show that uvomorulin is synthesized as a precursor polypeptide (Mr 135,000) that is core glycosylated in the endoplasmic reticulum. The precursor is processed to the mature polypeptide (Mr 120,000) shortly after addition of complex carbohydrate groups in the late Golgi complex, but prior to delivery of the polypeptide to the cell surface. However, glycosylation is not required for either efficient processing of the precursor or transport of uvomorulin to the cell surface. At the cell surface, uvomorulin is turned over rapidly (t1/2 approximately 5 h). Induction of Ca(2+)-dependent cell-cell contact results in rapid localization of cell surface uvomorulin to regions of contact and an increase in the proportion of uvomorulin that is insoluble in buffers containing Triton X-100. These results indicate several regulatory steps in the biosynthesis and cell surface expression of uvomorulin in epithelial cells.