Homoeologous recombination-based transfer and molecular cytogenetic mapping of a wheat streak mosaic virus and Triticum mosaic virus resistance gene Wsm3 from Thinopyrum intermedium to wheat
Key message Here, we report the production of a wheat– Thinopyrum intermedium recombinant stock conferring resistance to wheat streak mosaic virus and Triticum mosaic virus. Wheat streak mosaic caused by the wheat streak mosaic virus (WSMV) is an important disease of bread wheat ( Triticum aestivum...
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Published in | Theoretical and applied genetics Vol. 130; no. 3; pp. 549 - 556 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.03.2017
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
Here, we report the production of a wheat–
Thinopyrum intermedium
recombinant stock conferring resistance to wheat streak mosaic virus and
Triticum
mosaic virus.
Wheat streak mosaic caused by the wheat streak mosaic virus (WSMV) is an important disease of bread wheat (
Triticum aestivum
) worldwide. To date, only three genes conferring resistance to WSMV have been named and two,
Wsm1
and
Wsm3
, were derived from the distantly related wild relative
Thinopyrum intermedium
.
Wsm3
is only available in the form of a compensating wheat–
Th. intermedium
whole-arm Robertsonian translocation T7BS·7S#3L. Whole-arm alien transfers usually suffer from linkage drag, which prevents their use in cultivar improvement. Here, we report
ph1b
-induced homoeologous recombination to shorten the
Th. intermedium
segment and recover a recombinant chromosome consisting of the short arm of wheat chromosome 7B, part of the long arm of 7B, and the distal 43% of the long arm derived from the
Th. intermedium
chromosome arm 7S#3L. The recombinant chromosome T7BS·7BL-7S#3L confers resistance to WSMV at 18 and 24 °C and also confers resistance to
Triticum
mosaic virus, but only at 18 °C.
Wsm3
is the only gene conferring resistance to WSMV at a high temperature level of 24 °C. We also developed a user-friendly molecular marker that will allow to monitor the transfer of
Wsm3
in breeding programs.
Wsm3
is presently being transferred to adapted hard red winter wheat cultivars and can be used directly in wheat improvement. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-016-2834-8 |