Biosynthesis of Fatty Acid Derivatives by Recombinant Yarrowia lipolytica Containing MsexD2 and MsexD3 Desaturase Genes from Manduca sexta

• Published in: Journal of fungi (Basel) Vol. 9; no. 1; p. 114
• Main Authors: Hambalko, Jaroslav, Gajdoš, Peter, Nicaud, Jean-Marc, Ledesma-Amaro, Rodrigo, Tupec, Michal, Pichová, Iva, Čertík, Milan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.01.2023; MDPI
• Subjects: Amino acids; Biosynthesis; conjugase; Desaturase; Enzymes; Esters; Fatty acid methyl esters; Fatty acids; Fermentation; Genes; Genomes; Genotypes; Glucose; Life Sciences; Linoleic acid; Linolenic acid; Lipids; Manduca; Manduca sexta; metabolic engineering; Microbiology and Parasitology; Microorganisms; pheromone; Plasmids; Sex pheromone; Yarrowia; Yeast; Slovakia; Belgium; United States > US; Germany; Yarrowia; conjugase; Manduca; desaturase; metabolic engineering; pheromone; desaturase Manduca
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof9010114

One of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids from which the most researched include: conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixtures of some insect species, including tobacco horn-worm ( ), are typical for the production of uncommon C16 long conjugated fatty acids with two and three conjugated double bonds, as opposed to C18 long CLA and CLNA. In this study, desaturases MsexD2 and MsexD3 were expressed in multiple strains of with different genotypes. Experiments with the supplementation of fatty acid methyl esters into the medium resulted in the production of novel fatty acids. Using GCxGC-MS, 20 new fatty acids with two or three double bonds were identified. Fatty acids with conjugated or isolated double bonds, or a combination of both, were produced in trace amounts. The results of this study prove that is capable of synthesizing C16-conjugated fatty acids. Further genetic optimization of the genome and optimization of the fermentation process could lead to increased production of novel fatty acid derivatives with biotechnologically interesting properties.