Calreticulin expression in neoplastic versus normal dog mammary glands: A cDNA subtraction-based study

• Published in: Research in veterinary science Vol. 92; no. 1; pp. 80 - 91
• Main Authors: Okawa, Takumi, Kurio, Yuzo, Morimoto, Masahiro, Hayashi, Toshiharu, Nakagawa, Takayuki, Sasaki, Nobuo, Okuda, Masaru, Mizuno, Takuya
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.02.2012; Elsevier Limited
• Subjects: adenocarcinoma; Adenocarcinoma - metabolism; Adenocarcinoma - veterinary; alpha-Macroglobulins; alpha-Macroglobulins - metabolism; amino acid sequences; analysis; Animals; Base Sequence; Biomarkers, Tumor; Biomarkers, Tumor - metabolism; Blotting, Western; Blotting, Western - veterinary; Breast cancer; Calreticulin; Calreticulin - genetics; Calreticulin - metabolism; Canine; carboxypeptidase C; Cathepsin A; Cathepsin A - metabolism; cDNA subtraction; Cell Line, Tumor; complementary DNA; Dog Diseases; Dog Diseases - metabolism; Dogs; Female; gene expression; gene expression regulation; Gene Library; genetics; Lactoferrin; Lactoferrin - metabolism; Mammary gland tumours; mammary glands; Mammary Neoplasms, Animal; Mammary Neoplasms, Animal - metabolism; messenger RNA; metabolism; Molecular Sequence Data; protein synthesis; Proteins; Real-Time Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction - veterinary; RNA, Messenger; RNA, Messenger - analysis; Rodents; Tumors; veterinary; Veterinary medicine; Calreticulin; cDNA subtraction; Mammary gland tumours; Canine
• ISSN: 0034-5288; 1532-2661; 1532-2661
• DOI: 10.1016/j.rvsc.2010.10.028

This is the first report describing the expression of canine calreticulin (cCRT) in canine mammary gland tumour (MGT). Using cDNA subtraction method, it is found that mRNAs of CRT, cathepsin A, ovostatin, and lactotransferrin were differentially expressed in mammary adenocarcinoma as compared to hyperplasia, both of which were obtained from the dog. Furthermore, the mRNA expression levels of CRT and cathepsin A were significantly higher in canine MGT samples than in nontumour samples. In contrast, immunohistochemical studies have indicated that the expression of cCRT protein found to be detected in most of mammary gland tissues and was not correlated to the types of canine MGTs. Furthermore, cCRT was molecularly cloned, and the amino acid sequence of cCRT was found to be very similar to those of other species. Further studies are required to elucidate additional roles of cCRT in canine MGT.