Natural variations in stearoyl-acp desaturase genes affect the conversion of stearic to oleic acid in maize kernel
Key message We identified 11 SAD genes, and mined their natural variations associated with the conservation of stearic to oleic acid, especially ZmSAD1 supported by both the QTL and an expression QTL. Maize oil is generally regarded as a healthy vegetable oil owing to its low abundance of saturated...
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Published in | Theoretical and applied genetics Vol. 130; no. 1; pp. 151 - 161 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
2017
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
We identified 11 SAD genes, and mined their natural variations associated with the conservation of stearic to oleic acid, especially
ZmSAD1
supported by both the QTL and an expression QTL.
Maize oil is generally regarded as a healthy vegetable oil owing to its low abundance of saturated fatty acids. Stearoyl-ACP desaturase (SAD) is a key rate-limiting enzyme for the conservation of stearic (C18:0) to oleic (C18:1) acid. Here, 11 maize SAD genes were identified to have more divergent functions than
Arabidopsis
SAD genes. The genomic regional associations in a maize panel including 508 inbred lines identified 6 SAD genes significantly associated (
P
< 0.01) with the C18:0/C18:1 ratio or the level of C18:0 or C18:1, one gene of which co-localized with a quantitative trait locus (QTL) and 5 of which co-localized with an expression QTL.
ZmSAD1
, supported by both the QTL and an expression QTL, had the largest effect on C18:0/C18:1. One nonsynonymous single-nucleotide polymorphism in exon 3 and one 5-bp insertion/deletion in the 3′ untranslated region were further shown to contribute to the natural variation in C18:0/C18:1 according to
ZmSAD1
-based association mapping. Finally, selection tests of
ZmSAD1
in teosinte, regular maize, and high-oil maize indicated that
ZmSAD1
was not a selection target during the process of maize domestication and high-oil maize development. These results will guide the manipulation of the ratio between saturated and unsaturated fatty acids in maize. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-016-2800-5 |