Loss of FAM60A attenuates cell proliferation in glioma via suppression of PI3K/Akt/mTOR signaling pathways

• Published in: Translational oncology Vol. 14; no. 11; p. 101196
• Main Authors: Liu, Xiaocen, Zhang, Mengying, Zhu, Xiaolong, Wang, Yingying, Lv, Kun, Yang, Hui
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.11.2021; Neoplasia Press; Elsevier
• Subjects: Biomarker; Cell proliferation; FAM60A; Glioma; Original Research; PI3K/Akt/mTOR; Cell proliferation; Biomarker; HEB; OS; IHC; PKB; qRT-PCR; MAPK; PI3K/Akt/mTOR; TCGA; DFS; Glioma; PI3K; NC; FBS; PtdIns; FAM60A; WHO; HDAC
• ISSN: 1936-5233; 1936-5233
• DOI: 10.1016/j.tranon.2021.101196

•FAM60A expression was significantly upregulated in glioma tissues and cell lines, and positively associated with a worse outcome in glioma.•FAM60A regulated glioma cell proliferation via suppression of PI3K/Akt/mTOR signaling pathways.•FAM60A expression is detectable in extracted serum exosomes and is more highly expressed in the glioma cancer group than in the normal group.•FAM60A may act as a prognostic biomarker and therapeutic target for glioma. Glioma is a common malignant tumor of the central nervous system with a high incidence and mortality. Family with sequence similarity 60 member A (FAM60A) is a new subunit of the Sin3 deacetylase complex. The clinical significance and biologic role of FAM60A in glioma remain unclear. The expression of FAM60A in normal glial cells, glioma cells, and five-paired gliomas, and adjacent noncancerous tissues was quantified using real-time polymerase chain reaction (PCR) and western blotting. FAM60A protein expression in 179 archived, paraffin-embedded glioma samples was analyzed using immunohistochemistry. The roles of FAM60A in glioma cell proliferation and tumorigenicity were explored in vitro and in vivo. The underlying molecular mechanisms were elucidated using Western blot assay. Serum exosomal FAM60A levels of glioma patients were detected using electron microscopy, western blot, and real-time PCR. FAM60A expression was significantly up-regulated in glioma tissues and cell lines and positively associated with a worse outcome in glioma. Knockdown of FAM60A could inhibit glioma cell proliferation and tumorigenicity in vitro and in vivo. Besides, FAM60A expression was detectable in extracted serum exosomes with a higher expression in the glioma cancer group than in the normal group. Loss of FAM60A attenuates cell proliferation in glioma by suppressing PI3K/Akt/mTOR signaling pathways. Therefore, FAM60A may act as a prognostic biomarker and therapeutic target for glioma.