The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke

• Published in: Nature communications Vol. 2; no. 1; p. 485
• Main Authors: Nakano, Kazuhiko, Hokamura, Kazuya, Taniguchi, Naho, Wada, Koichiro, Kudo, Chiho, Nomura, Ryota, Kojima, Ayuchi, Naka, Shuhei, Muranaka, Yoshinori, Thura, Min, Nakajima, Atsushi, Masuda, Katsuhiko, Nakagawa, Ichiro, Speziale, Pietro, Shimada, Nobumitsu, Amano, Atsuo, Kamisaki, Yoshinori, Tanaka, Tokutaro, Umemura, Kazuo, Ooshima, Takashi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27.09.2011; Nature Publishing Group
• Subjects: 631/326/41/2531; 692/308; 692/699/375/1370/534; Animals; Bacterial Proteins - metabolism; Cerebral Hemorrhage - metabolism; Collagen - metabolism; Humanities and Social Sciences; Mice; multidisciplinary; Platelet Aggregation; Rats; Rats, Inbred SHR; Risk factors; Science; Science (multidisciplinary); Streptococcus mutans - metabolism; Streptococcus mutans - pathogenicity; Stroke - metabolism; Virulence
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms1491

Although several risk factors for stroke have been identified, one-third remain unexplained. Here we show that infection with Streptococcus mutans expressing collagen-binding protein (CBP) is a potential risk factor for haemorrhagic stroke. Infection with serotype k S. mutans , but not a standard strain, aggravates cerebral haemorrhage in mice. Serotype k S. mutans accumulates in the damaged, but not the contralateral hemisphere, indicating an interaction of bacteria with injured blood vessels. The most important factor for high-virulence is expression of CBP, which is a common property of most serotype k strains. The detection frequency of CBP-expressing S. mutans in haemorrhagic stroke patients is significantly higher than in control subjects. Strains isolated from haemorrhagic stroke patients aggravate haemorrhage in a mouse model, indicating that they are haemorrhagic stroke-associated. Administration of recombinant CBP causes aggravation of haemorrhage. Our data suggest that CBP of S. mutans is directly involved in haemorrhagic stroke. The risk factors associated with both ischemic and haemorrhagic stroke are not fully understood. Here a certain strain of the bacteria, Streptococcus mutans , which expresses a collagen-binding protein, is shown to be associated with haemorrhagic stroke in both animal models and human patients.