pH-Dependent accumulation of anticancer compound on mycelia in fermentation of marine fungus

• Published in: Journal of industrial microbiology & biotechnology Vol. 41; no. 7; pp. 1169 - 1173
• Main Authors: Zhou, Weiqiang, Cai, Menghao, Na, Ke, Shen, Chu, Zhang, Xiaoxu, Zhou, Xiangshan, Zhao, Wenjie, Zhang, Yuanxing
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.07.2014; Springer Berlin Heidelberg; Springer; Oxford University Press
• Subjects: Analysis; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - metabolism; Aquatic Organisms - growth & development; Aquatic Organisms - metabolism; Aquatic Organisms - ultrastructure; Biochemistry; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Bioreactors - microbiology; Biotechnology; Cancer; Cancer therapies; Electron microscopes; Fermentation; Fractional Precipitation; Fundamental and applied biological sciences. Psychology; Fungi; Genetic Engineering; Glucose; Hydrogen-Ion Concentration; Inorganic Chemistry; Life Sciences; marine fungi; Metabolism; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbiology; Morphology; mycelium; Mycelium - growth & development; Mycelium - metabolism; Mycelium - ultrastructure; Pharmaceuticals; scanning electron microscopes; Scanning electron microscopy; Short Communication; Studies; Xylariales - growth & development; Xylariales - metabolism; Xylariales - ultrastructure; pH-Dependent; Marine; sp; Anticancer 1403C; Product distribution; Antineoplastic agent; Fungi; Marine environment; pH; Marine Halorosellinia sp; Fermentation; Accumulation
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-014-1445-0

The real-time distribution of anticancer 1403C in fermentation broth of marine fungus Halorosellinia sp. was investigated. It was closely related with pH variations, which was, 1403C in the supernatant decreased while that in the mycelia increased with pH rising. There was only 0.5 % of the total 1403C left in the supernatant when pH reached 7.0. The scanning electron microscope then provided information that compounds precipitated on the mycelia when pH rose. Then, the pH-regulation experiments proved that 1403C mainly secreted extracellular and easily dissolved in acidic condition but precipitated and absorbed on the mycelia with the increase of broth pH. Thereby, a pH-regulation strategy was proposed and applied to accumulate 1403C on the mycelia before draw-off of fermentation broth. It significantly simplified purification process and is critical for 1403C preparation of industrial scale.