TXM13 human melanoma cells: a novel source for the inhibition kinetics of human tyrosinase and for screening whitening agents

• Published in: Biochemistry and cell biology Vol. 84; no. 1; pp. 112 - 116
• Main Authors: Park, Yong-Doo, Kim, So-yeon, Lyou, You-Jeong, Lee, Dong-Youn, Yang, Jun-Mo
• Format: Journal Article
• Language: English
• Published: Ottawa, Canada NRC Research Press 01.02.2006; Canadian Science Publishing NRC Research Press
• Subjects: Binding sites; Cancer cells; Cells; Enzyme Inhibitors - pharmacology; Genetic aspects; Glycerol - pharmacology; Humans; Hypopigmentation; Kinetics; Melanoma; Melanoma - enzymology; Monophenol Monooxygenase - antagonists & inhibitors; Monophenol Monooxygenase - metabolism; Mutation; Phenotype; Physiological aspects; Proteins; Skin Pigmentation - drug effects; Tyrosine; United States
• ISSN: 0829-8211; 1208-6002
• DOI: 10.1139/o05-151

Research involving whitening agents requires several steps of experimentation, and the initial step is to test whitening agents with human melanocytes and those with human tyrosinase. Unfortunately, it takes a long time to gather human melanocytes, and these cells have some limitations when it comes to performing experiments, such as their passage difficulties and their cost. In this study, we suggest that the TXM13 human melanoma cells could be a useful cell candidate for studying human tyrosinase inhibition and depigmentation. We applied a tyrosinase inhibitor, such as dithioglycerine (DTGC), to validate the cell line's usefulness, and we tested the effect of DTGC on TXM13 melanogenesis. The results showed that human tyrosinase from TXM13 was appropriate, according to the inhibition kinetics, and that the conspicuous depigmentation of TXM13 occurred after DTGC treatment without downregulating the tyrosinase expression level. When taken together, our findings provide useful information regarding the use of the TXM13 melanoma cells for the development of whitening agents.Key words: TXM13, pigment cell, human tyrosinase, whitening agent.