CYP76C2, an Arabidopsis thaliana cytochrome P450 gene expressed during hypersensitive and developmental cell death

• Published in: FEBS letters Vol. 438; no. 3; pp. 245 - 249
• Main Authors: Godiard, Laurence, Sauviac, Laurent, Dalbin, Nathalie, Liaubet, Laurence, Callard, Didier, Czernic, Pierre, Marco, Yves
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 06.11.1998; Wiley
• Subjects: ABA, abscissic acid; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - microbiology; Bacterial leaf spot; Cloning, Molecular; Col-0, Columbia; Cytochrome P-450 Enzyme System - genetics; Defence gene; EST, expressed sequence tags; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; hpi, hours post inoculation; HR, hypersensitive response; hsr genes, hypersensitivity-related genes; Hypersensitive response; Life Sciences; Multigene Family; P450, cytochrome P450; Plant Leaves; Pseudomonas - genetics; Pseudomonas - pathogenicity; Pseudomonas - physiology; Senescence; Virulence; Defence gene; Col-0, Columbia; Senescence; Hypersensitive response; hsr genes, hypersensitivity-related genes; EST, expressed sequence tags; hpi, hours post inoculation; Bacterial leaf spot; HR, hypersensitive response; P450, cytochrome P450; ABA, abscissic acid; REPONSE DE LA PLANTE
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(98)01309-X

The characterisation of an Arabidopsis thaliana cytochrome P450-encoding cDNA clone, B72, preferentially expressed during the hypersensitive response (HR) provoked by the bacterial pathogen Pseudomonas syringae pathovar maculicola, is reported. The B72 cDNA clone corresponded to the CYP76C2 gene, which belongs to a small multigene family comprising four genes. HR-triggering bacteria harbouring different avirulence genes induced the accumulation of transcripts of this P450 gene. CYP76C2 gene expression was moreover associated with various processes leading to cell death such as leaf senescence, ageing of cell cultures, wounding as well as with treatment with the necrotising heavy metal salt, lead nitrate.