The labelling index is not always reliable. Discrepancies between the labelling index and the mitotic rate in the rat corneal epithelium after intraperitoneal and topical administration of tritiated thymidine and colcemid

Many cell kinetic studies are based on the assumption that tritiated thymidine injected into an animal is available for incorporation into DNA for only a short time, and that it labels all cells in the S phase. The present study indicates that this is not the fact for the rat corneal epithelium. The...

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Bibliographic Details
Published inCell and tissue kinetics Vol. 21; no. 6; p. 389
Main Authors Haaskjold, E, Refsum, S B, Bjerknes, R, Paulsen, T O
Format Journal Article
LanguageEnglish
Published England 01.11.1988
Subjects
Administration, Topical
Animals
Cornea - cytology
Cornea - drug effects
Cytological Techniques
Demecolcine - administration & dosage
Demecolcine - pharmacology
Epithelial Cells
Epithelium - drug effects
Female
Injections, Intraperitoneal
Interphase
Mitosis - drug effects
Rats
Rats, Inbred Strains
Thymidine - administration & dosage
Thymidine - pharmacology
Tritium
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Summary:Many cell kinetic studies are based on the assumption that tritiated thymidine injected into an animal is available for incorporation into DNA for only a short time, and that it labels all cells in the S phase. The present study indicates that this is not the fact for the rat corneal epithelium. The labelling index (LI) declines considerably from the limbal area to the central cornea, while the mitotic rate is almost constant all over the corneal epithelium. The LI should therefore not be used as the only criterion in the assessment of proliferation rate.
ISSN:0008-8730
DOI:10.1111/j.1365-2184.1988.tb00798.x