Characterization of bixin by LC-MS and LC-NMR

• Published in: Journal of separation science Vol. 30; no. 15; pp. 2382 - 2390
• Main Authors: Rehbein, Jens, Dietrich, Benjamin, Grynbaum, Marc David, Hentschel, Petra, Holtin, Karsten, Kuehnle, Maximilian, Schuler, Paul, Bayer, Marc, Albert, Klaus
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.10.2007; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley
• Subjects: Acetone - analysis; Analytical chemistry; Bixin stereoisomers; Capillary LC-NMR; Carotenoids; Carotenoids - analysis; Carotenoids - chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Chromatography, Liquid - methods; Equipment Design; Exact sciences and technology; Free Radicals; LC-MS; Magnetic Resonance Spectroscopy - methods; Mass Spectrometry - methods; Models, Chemical; Other chromatographic methods; Plant Extracts - analysis; Plant Extracts - chemistry; Protein Isoforms; Capillary column; Stereoisomer; Bixin stereoisomers; Separation; Atmospheric pressure; Bixin; Natural dye; Coupled method; COSY sequence; HPLC chromatography; NMR spectrometry; Chemical ionization; Two dimension spectrometry; Hydrogen 1; Capillary LC-NMR; Reversed phase chromatography; Chemical shift; Stopped flow method; LC-MS; Carotenoids; Carotenoid; Mass spectrometry; Structural analysis
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200700089

An overview upon modern analytical techniques for the isolation, separation, and structural identification of the essential bioactive carotenoid bixin is given. Isolation from biological matrices is performed by matrix solid phase dispersion (MSPD). The extract is separated with shape-selective C₃₀ columns. Structural assignment of the separated compounds is done by online LC-MS and capillary HPLC-NMR.