Characterization and quantification of triacylglycerols in peanut oil by off-line comprehensive two-dimensional liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry

• Published in: Journal of separation science Vol. 36; no. 2; pp. 288 - 300
• Main Authors: Hu, Jun, Wei, Fang, Dong, Xu-Yan, Lv, Xin, Jiang, Mu-Lan, Li, Guang-Ming, Chen, Hong
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.01.2013; Wiley; Wiley Subscription Services, Inc
• Subjects: Atmospheric pressure; Atmospheric pressure chemical ionization mass spectrometry; Biological and medical sciences; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Comprehensive two-dimensional liquid chromatography; Fat industries; Food industries; Fundamental and applied biological sciences. Psychology; Ions; Mass spectrometry; Mass Spectrometry - methods; Nuts; Oils & fats; Peanut Oil; Plant Oils - chemistry; Reversed-phase liquid chromatography; Scientific imaging; Silver-ion liquid chromatography; Triacylglycerols; Triglycerides - analysis; Tandem mass spectrometry; Chemical analysis; Two dimensional chromatography; Atmospheric pressure; Coupled method; HPLC chromatography; Lipids; Vegetable oil; Atmospheric pressure chemical ionization mass spectrometry; Reversed-phase liquid chromatography; Edible oil; Comprehensive two-dimensional liquid chromatography; Glyceride; Chemical ionization; Reversed phase chromatography; Position isomer; Triacylglycerol; Silver-ion liquid chromatography; Off line; Silver ion; Non aqueous solvent; Triacylglycerols; Quantitative analysis; Peanut oil
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201200567

The complexity of natural triacylglycerols (TAGs) in various edible oils is prodigious due to the hundreds of set is of TAG compositions, which makes the identification of TAGs quite difficult. In this investigation, the off‐line 2D system coupling of nonaqueous RP and silver‐ion HPLC with atmospheric pressure chemical ionization MS detection has been applied to the identification and quantification of TAGs in peanut oil. The method was successful in the separation of a high number of TAG solutes, and the TAG structures were evaluated by analyzing their atmospheric pressure chemical ionization mass spectra information. HPLC and MS conditions have been optimized and the fragmentation mechanisms of isomers have been validated. In addition, an internal standard approach has been developed for TAG quantification. Then this system was applied in peanut oil samples and there was a total of 48 TAGs including regioisomers that have been determined and quantified.