Human mitochondrial peroxiredoxin 5 protects from mitochondrial DNA damages induced by hydrogen peroxide

• Published in: FEBS letters Vol. 579; no. 11; pp. 2327 - 2333
• Main Authors: Banmeyer, Ingrid, Marchand, Cécile, Clippe, André, Knoops, Bernard
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 25.04.2005
• Subjects: Animals; Antioxidant enzyme; C47S-PRDX5; CCC; Chinese hamster ovary cells; CHO Cells; COX1; Cricetinae; cytochrome C oxidase subunit 1; dithiothreitol; DNA Damage - drug effects; DNA, Mitochondrial - metabolism; DTT; H2O2; Humans; hydrogen peroxide; Hydrogen Peroxide - pharmacology; hydroxyl radical; MFO; Mitochondria; Mitochondria - drug effects; Mitochondria - genetics; mitochondrial DNA; mtDNA; NADH dehydrogenase subunit 4 gene; ND4; nDNA; NLS; nuc-PRDX5; nuclear DNA; nuclear human recombinant PRDX5; nuclear localization signal; OH; open circular plasmid DNA; Oxidation-Reduction - drug effects; Oxidative Stress - drug effects; Peroxidases - genetics; Peroxidases - metabolism; Peroxide; Peroxiredoxin; Peroxiredoxins; Plasmids - genetics; PRDX; reactive oxygen species; recombinant C47S mutant of human PRDX5; Reference Standards; RNA, Messenger - genetics; ROS; supercoiled plasmid DNA; tBHP; tert-butylhydroperoxide; thiol/Fe3+/O2 mixed-function oxidase; Thioredoxin peroxidase; wild-type human recombinant PRDX5; wt-PRDX5; Peroxiredoxin; CCC; C47S-PRDX5; tBHP; DTT; mtDNA; Antioxidant enzyme; H2O2; Mitochondria; PRDX; Peroxide; NLS; OC; MFO; ROS; Thioredoxin peroxidase; OH; wt-PRDX5; COX1; nDNA; CHO cells; ND4; nuc-PRDX5
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2005.03.027

Peroxiredoxin 5 is a thioredoxin peroxidase ubiquitously expressed in mammalian tissues. Peroxiredoxin 5 can be addressed intracellularly to mitochondria, peroxisomes, the cytosol and the nucleus. Here, we show that mitochondrial human peroxiredoxin 5 protects mitochondrial DNA (mtDNA) from oxidative attacks. In an acellular assay, recombinant peroxiredoxin 5 was shown to protect plasmid DNA from damages induced by metal-catalyzed generation of reactive oxygen species. In Chinese hamster ovary cells, overexpression of mitochondrial peroxiredoxin 5 significantly decreased mtDNA damages caused by exogenously added hydrogen peroxide. Altogether our results suggest that mitochondrial peroxiredoxin 5 may play an important role in mitochondrial genome stability.