Cloning and functional analysis by gene disruption of a novel gene involved in indigo production and fluoranthene metabolism in Pseudomonas alcaligenes PA-10

• Published in: FEMS microbiology letters Vol. 239; no. 2; pp. 285 - 293
• Main Authors: Alemayehu, D., Gordon, L.M., O’Mahony, M.M., O’Leary, N.D., Dobson, A.D.W.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 15.10.2004; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Acyl-CoA dehydrogenase; Alcaligenes; Bacteriological methods and techniques used in bacteriology; Bacteriology; biodegradation; Biological and medical sciences; biosynthesis; Cloning; Cloning, Molecular; Dioxygenase; Dioxygenases - metabolism; Disruption; Fluoranthene; Fluorenes - metabolism; Functional analysis; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene disruption; Gene expression; Gene Expression Regulation, Bacterial; Genes, Bacterial - physiology; Genetics; idoA gene; Indigo; Indigo Carmine; Indole dioxygenase; Indoles; Indoles - metabolism; Intermediates; Metabolism; Microbiology; Naphthalene; Novosphingobium aromaticivorans; Nucleotides; oxygenases; Phylogeny; Polycyclic aromatic hydrocarbons; Pseudomonas; Pseudomonas alcaligenes; Pseudomonas alcaligenes - genetics; Pseudomonas alcaligenes - metabolism; Salicylic acid; structural genes; Pseudomonas alcaligenes; Polycyclic aromatic hydrocarbons; Indole dioxygenase; Fluoranthene; Pseudomonadales; Polymerase chain reaction; Gene; Hydrocarbon; Phylogenetic tree; Bacteria; Pseudomonadaceae; Sequence; Polycyclic aromatic compound; Metabolism; Reverse transcription polymerase chain reaction
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2004.08.046

A novel indole dioxygenase ( idoA) gene has been cloned from Pseudomonas alcaligenes PA-10, based on its ability to convert indole to indigo. The chromosomally encoded idoA gene exhibits no similarity to previously cloned naphthalene dioxygenases or to aromatic oxygenases from other species at the nucleotide level. Phylogenetic analysis indicates that the idoA gene product is most similar to an acyl-CoA dehydrogenase from Novosphingobium aromaticivorans. The enzyme encoded by the idoA gene is essential for the metabolism of fluoranthene, since a mutant in which the idoA gene has been disrupted looses the ability to degrade this compound. The idoA gene appears to be constitutively expressed in PA-10, but its expression is also subject to regulation following prior exposure to salicylate and to fluoranthene degradative intermediates.